Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection

Chun Kyon Lee, Jeong Hun Suh, Young Suk Cho, KwangHyub Han, Jae Bock Chung, Chae Yoon Chon, Young Myoung Moon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

BACKGROUND/AIMS: The protective role of HBV-specific CD8+ cells is dependent on their ability to efficiently migrate to the infected liver, where they may exert an effector function. The migratory behavior of CD8+ cells is influenced by their expression of different chemokine receptors. This study was intended to analyse the pattern of chemokine receptor expression of HBV specific CD 8+ cells in chronic B viral infection. METHODS: We analysed the CCR5 and CCR3 profile of HBV-specific CD8+ cells isolated from the blood and liver of patients with different patterns of HBV infection. Purified T cells were stained directly ex vivo, or after antigen-specific stimulation, using HBV peptide-specific HLA tetramers and monoclonal antibodies to CD8, CCR5 and CCR3, with analysis by flow cytometry. RESULTS: In patients with chronic hepatitis B characterised by low levels of virus (serum HBV DNA <0.5 pg/mL) and minimal liver inflammation, analysis of circulating and intrahepatic CD8+ cells demonstrated that liver infiltrating Tc18-27-specific cells were preferentially CCR5+ (up to 80% of HBV-specific CD8+ cells), in contrast to cells of the same specificity within the circulating compartment (up to 35% of HBV-specific CD8+ cells). Furthermore, CCR3 was expressed by about 10% of Tc18-27+ cells infiltrating the liver, but was absent from circulating cells. Following HBV-specific stimulation in vitro the CCR5 expression of circulating Tc18-27-specific cells was up-regulated, to levels found in liver infiltrating cells, whereas CCR3 expression was unchanged. CONCLUSIONS: The chemokine receptor profile of HBV-specific CD8+ cells is influenced by the anatomical site of these cells, and the clinical pattern of disease. The ability of circulating HBV-specific CD8+ cells of patients with low replicating virus to upregulate CCR5 suggests that these cells may respond to increases in virus replication by efficiently migrating into the infected liver.

Original languageEnglish
Pages (from-to)363-370
Number of pages8
JournalTaehan Kan Hakhoe chi = The Korean journal of hepatology
Volume8
Issue number4
Publication statusPublished - 2002 Jan 1

Fingerprint

Chemokine Receptors
Virus Diseases
Hepatitis B virus
Lymphocytes
Liver
Viruses
Chronic Hepatitis B
Virus Replication

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, Chun Kyon ; Suh, Jeong Hun ; Cho, Young Suk ; Han, KwangHyub ; Chung, Jae Bock ; Chon, Chae Yoon ; Moon, Young Myoung. / Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection. In: Taehan Kan Hakhoe chi = The Korean journal of hepatology. 2002 ; Vol. 8, No. 4. pp. 363-370.
@article{f9d9697ba18b42ddb2f7aa46110ac6ba,
title = "Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection",
abstract = "BACKGROUND/AIMS: The protective role of HBV-specific CD8+ cells is dependent on their ability to efficiently migrate to the infected liver, where they may exert an effector function. The migratory behavior of CD8+ cells is influenced by their expression of different chemokine receptors. This study was intended to analyse the pattern of chemokine receptor expression of HBV specific CD 8+ cells in chronic B viral infection. METHODS: We analysed the CCR5 and CCR3 profile of HBV-specific CD8+ cells isolated from the blood and liver of patients with different patterns of HBV infection. Purified T cells were stained directly ex vivo, or after antigen-specific stimulation, using HBV peptide-specific HLA tetramers and monoclonal antibodies to CD8, CCR5 and CCR3, with analysis by flow cytometry. RESULTS: In patients with chronic hepatitis B characterised by low levels of virus (serum HBV DNA <0.5 pg/mL) and minimal liver inflammation, analysis of circulating and intrahepatic CD8+ cells demonstrated that liver infiltrating Tc18-27-specific cells were preferentially CCR5+ (up to 80{\%} of HBV-specific CD8+ cells), in contrast to cells of the same specificity within the circulating compartment (up to 35{\%} of HBV-specific CD8+ cells). Furthermore, CCR3 was expressed by about 10{\%} of Tc18-27+ cells infiltrating the liver, but was absent from circulating cells. Following HBV-specific stimulation in vitro the CCR5 expression of circulating Tc18-27-specific cells was up-regulated, to levels found in liver infiltrating cells, whereas CCR3 expression was unchanged. CONCLUSIONS: The chemokine receptor profile of HBV-specific CD8+ cells is influenced by the anatomical site of these cells, and the clinical pattern of disease. The ability of circulating HBV-specific CD8+ cells of patients with low replicating virus to upregulate CCR5 suggests that these cells may respond to increases in virus replication by efficiently migrating into the infected liver.",
author = "Lee, {Chun Kyon} and Suh, {Jeong Hun} and Cho, {Young Suk} and KwangHyub Han and Chung, {Jae Bock} and Chon, {Chae Yoon} and Moon, {Young Myoung}",
year = "2002",
month = "1",
day = "1",
language = "English",
volume = "8",
pages = "363--370",
journal = "Clinical and molecular hepatology",
issn = "2287-2728",
publisher = "Korean Association for the Study of the Liver",
number = "4",

}

Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection. / Lee, Chun Kyon; Suh, Jeong Hun; Cho, Young Suk; Han, KwangHyub; Chung, Jae Bock; Chon, Chae Yoon; Moon, Young Myoung.

In: Taehan Kan Hakhoe chi = The Korean journal of hepatology, Vol. 8, No. 4, 01.01.2002, p. 363-370.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemokine receptor expression of hepatitis B virus-specific CD8+ lymphocyte in chronic B viral infection

AU - Lee, Chun Kyon

AU - Suh, Jeong Hun

AU - Cho, Young Suk

AU - Han, KwangHyub

AU - Chung, Jae Bock

AU - Chon, Chae Yoon

AU - Moon, Young Myoung

PY - 2002/1/1

Y1 - 2002/1/1

N2 - BACKGROUND/AIMS: The protective role of HBV-specific CD8+ cells is dependent on their ability to efficiently migrate to the infected liver, where they may exert an effector function. The migratory behavior of CD8+ cells is influenced by their expression of different chemokine receptors. This study was intended to analyse the pattern of chemokine receptor expression of HBV specific CD 8+ cells in chronic B viral infection. METHODS: We analysed the CCR5 and CCR3 profile of HBV-specific CD8+ cells isolated from the blood and liver of patients with different patterns of HBV infection. Purified T cells were stained directly ex vivo, or after antigen-specific stimulation, using HBV peptide-specific HLA tetramers and monoclonal antibodies to CD8, CCR5 and CCR3, with analysis by flow cytometry. RESULTS: In patients with chronic hepatitis B characterised by low levels of virus (serum HBV DNA <0.5 pg/mL) and minimal liver inflammation, analysis of circulating and intrahepatic CD8+ cells demonstrated that liver infiltrating Tc18-27-specific cells were preferentially CCR5+ (up to 80% of HBV-specific CD8+ cells), in contrast to cells of the same specificity within the circulating compartment (up to 35% of HBV-specific CD8+ cells). Furthermore, CCR3 was expressed by about 10% of Tc18-27+ cells infiltrating the liver, but was absent from circulating cells. Following HBV-specific stimulation in vitro the CCR5 expression of circulating Tc18-27-specific cells was up-regulated, to levels found in liver infiltrating cells, whereas CCR3 expression was unchanged. CONCLUSIONS: The chemokine receptor profile of HBV-specific CD8+ cells is influenced by the anatomical site of these cells, and the clinical pattern of disease. The ability of circulating HBV-specific CD8+ cells of patients with low replicating virus to upregulate CCR5 suggests that these cells may respond to increases in virus replication by efficiently migrating into the infected liver.

AB - BACKGROUND/AIMS: The protective role of HBV-specific CD8+ cells is dependent on their ability to efficiently migrate to the infected liver, where they may exert an effector function. The migratory behavior of CD8+ cells is influenced by their expression of different chemokine receptors. This study was intended to analyse the pattern of chemokine receptor expression of HBV specific CD 8+ cells in chronic B viral infection. METHODS: We analysed the CCR5 and CCR3 profile of HBV-specific CD8+ cells isolated from the blood and liver of patients with different patterns of HBV infection. Purified T cells were stained directly ex vivo, or after antigen-specific stimulation, using HBV peptide-specific HLA tetramers and monoclonal antibodies to CD8, CCR5 and CCR3, with analysis by flow cytometry. RESULTS: In patients with chronic hepatitis B characterised by low levels of virus (serum HBV DNA <0.5 pg/mL) and minimal liver inflammation, analysis of circulating and intrahepatic CD8+ cells demonstrated that liver infiltrating Tc18-27-specific cells were preferentially CCR5+ (up to 80% of HBV-specific CD8+ cells), in contrast to cells of the same specificity within the circulating compartment (up to 35% of HBV-specific CD8+ cells). Furthermore, CCR3 was expressed by about 10% of Tc18-27+ cells infiltrating the liver, but was absent from circulating cells. Following HBV-specific stimulation in vitro the CCR5 expression of circulating Tc18-27-specific cells was up-regulated, to levels found in liver infiltrating cells, whereas CCR3 expression was unchanged. CONCLUSIONS: The chemokine receptor profile of HBV-specific CD8+ cells is influenced by the anatomical site of these cells, and the clinical pattern of disease. The ability of circulating HBV-specific CD8+ cells of patients with low replicating virus to upregulate CCR5 suggests that these cells may respond to increases in virus replication by efficiently migrating into the infected liver.

UR - http://www.scopus.com/inward/record.url?scp=2342593442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342593442&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 363

EP - 370

JO - Clinical and molecular hepatology

JF - Clinical and molecular hepatology

SN - 2287-2728

IS - 4

ER -