Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity

Hyo Young Kim, Seong Jae Kang, Yongmoon Jeon, Jinsu An, Jihyun Park, Hee Jae Lee, Jeong Eun Jang, Jong Seong Ahn, Duhee Bang, Hak Suk Chung, Cherlhyun Jeong, Dae Ro Ahn

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Abstract

We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.

Original languageEnglish
Pages (from-to)3552-3555
Number of pages4
JournalChemical Communications
Volume55
Issue number24
DOIs
Publication statusPublished - 2019 Jan 1

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All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Kim, H. Y., Kang, S. J., Jeon, Y., An, J., Park, J., Lee, H. J., Jang, J. E., Ahn, J. S., Bang, D., Chung, H. S., Jeong, C., & Ahn, D. R. (2019). Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity. Chemical Communications, 55(24), 3552-3555. https://doi.org/10.1039/c8cc08468h