We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.
Bibliographical noteFunding Information:
This work was supported by the Samsung Research Funding Center of Samsung Electronics under Project Number SRFC-MA1601-07 and a National Research Foundation of Korea (NRF) Grant (No. 2018R1A2B2008995).
© The Royal Society of Chemistry.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry