TY - JOUR
T1 - Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography
AU - Zbrozek, Jaroslav
AU - Pumera, Martin
AU - Flegel, Martin
PY - 2002/10
Y1 - 2002/10
N2 - Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.
AB - Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.
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U2 - 10.1093/chromsci/40.9.505
DO - 10.1093/chromsci/40.9.505
M3 - Article
C2 - 12433112
AN - SCOPUS:0036802638
VL - 40
SP - 505
EP - 508
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
SN - 0021-9665
IS - 9
ER -