Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography

Jaroslav Zbrozek, Martin Pumera, Martin Flegel

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4 Citations (Scopus)


Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.

Original languageEnglish
Pages (from-to)505-508
Number of pages4
JournalJournal of Chromatographic Science
Issue number9
Publication statusPublished - 2002 Oct


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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