Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy

W. Lee, E. Bang, W. Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalChromatographia
Volume57
Issue number7-8
DOIs
Publication statusPublished - 2003 Apr 1

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Crown Ethers
High performance liquid chromatography
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Enantiomers
High Pressure Liquid Chromatography
Chemical shift
Protons
Chemical analysis
diphenylalanine

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

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Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy. / Lee, W.; Bang, E.; Lee, W.

In: Chromatographia, Vol. 57, No. 7-8, 01.04.2003, p. 457-461.

Research output: Contribution to journalArticle

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