Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy

W. Lee; E. Bang; W. Lee

doi:10.1007/BF02492541

Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy

W. Lee, E. Bang, W. Lee

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.

Original language	English
Pages (from-to)	457-461
Number of pages	5
Journal	Chromatographia
Volume	57
Issue number	7-8
DOIs	https://doi.org/10.1007/BF02492541
Publication status	Published - 2003 Apr

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1007/BF02492541

Fingerprint Dive into the research topics of 'Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy'. Together they form a unique fingerprint.

Cite this

Lee, W., Bang, E., & Lee, W. (2003). Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy. Chromatographia, 57(7-8), 457-461. https://doi.org/10.1007/BF02492541

@article{a6198cb8620546adb7761d00e7f386e9,

title = "Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy",

abstract = "The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.",

author = "W. Lee and E. Bang and W. Lee",

year = "2003",

month = apr,

doi = "10.1007/BF02492541",

language = "English",

volume = "57",

pages = "457--461",

journal = "Chromatographia",

issn = "0009-5893",

publisher = "Springer Vieweg",

number = "7-8",

}

TY - JOUR

T1 - Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy

AU - Lee, W.

AU - Bang, E.

AU - Lee, W.

PY - 2003/4

Y1 - 2003/4

N2 - The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.

AB - The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.

UR - http://www.scopus.com/inward/record.url?scp=0037391609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037391609&partnerID=8YFLogxK

U2 - 10.1007/BF02492541

DO - 10.1007/BF02492541

M3 - Article

AN - SCOPUS:0037391609

VL - 57

SP - 457

EP - 461

JO - Chromatographia

JF - Chromatographia

SN - 0009-5893

IS - 7-8

ER -