Abstract
The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.
| Original language | English |
|---|---|
| Pages (from-to) | 457-461 |
| Number of pages | 5 |
| Journal | Chromatographia |
| Volume | 57 |
| Issue number | 7-8 |
| DOIs | |
| Publication status | Published - 2003 Apr 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Organic Chemistry
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Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy. / Lee, W.; Bang, E.; Lee, W.
In: Chromatographia, Vol. 57, No. 7-8, 01.04.2003, p. 457-461.Research output: Contribution to journal › Article
TY - JOUR
T1 - Chiral resolution of diphenylalanine by high-performance liquid chromatography on a crown-ether-based chiral stationary phase and by NMR spectroscopy
AU - Lee, W.
AU - Bang, E.
AU - Lee, W.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.
AB - The enantiomers of diphenylalanine (DPA) were well separated by chiral HPLC and NMR spectroscopy on the chiral stationary phase (CSP) derived from (18-crown-6)-2,3,11,12-tetracarboxylic acid (18-C-6-TA). The chromatographic parameters such as separation factors and retention times were greatly influenced by the mobile phase conditions. The (+)-18-C-6-TA used in the CSP was also employed as a chiral solvating agent for the enantiodiscrimination of the DPA enantiomers by NMR spectroscopy. The proton of the DPA analyte showing the chemical shift nonequivalences was used in determining the enantiomeric composition of the analyte.
UR - http://www.scopus.com/inward/record.url?scp=0037391609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037391609&partnerID=8YFLogxK
U2 - 10.1007/BF02492541
DO - 10.1007/BF02492541
M3 - Article
AN - SCOPUS:0037391609
VL - 57
SP - 457
EP - 461
JO - Chromatographia
JF - Chromatographia
SN - 0009-5893
IS - 7-8
ER -