Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord

So Jung Gwak; Jong Kwon Jung; Sung Su An; Hyo Jin Kim; Jin Soo Oh; William A. Pennant; Hye Yeong Lee; Min Ho Kong; Keung Nyun Kim; Do Heum Yoon; Yoon Ha

doi:10.1163/092050611X584090

Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord

So Jung Gwak, Jong Kwon Jung, Sung Su An, Hyo Jin Kim, Jin Soo Oh, William A. Pennant, Hye Yeong Lee, Min Ho Kong, Keung Nyun Kim, Do Heum Yoon, Yoon Ha

Department of Neurosurgery

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Gene delivery offers therapeutic promise for the treatment of neurological diseases and spinal cord injury. Several studies have offered viral vectors as vehicles to deliver therapeutic agents, yet their toxicity and immunogenicity, along with the cost of their large-scale formulation, limits their clinical use. As such, non-viral vectors are attractive in that they offer improved safety profiles compared to viruses. Poly(ethylene imine) (PEI) is one of the most extensively studied non-viral vectors, but its clinical value is limited by its cytotoxicity. Recently, chitosan/DNA complex nanoparticles have been considered as a vector for gene delivery. Here, we demonstrate that DNA nanoparticles made of hyaluronic acid (HA) and chitosan have low cytotoxicity and induce high transgene expression in neural stem cells and organotypic spinal cord slice tissue. Chitosan-TPP/HA nanoparticles were significantly less cytotoxic than PEI at various concentrations. Additionally, chitosan-TPP/HA nanoparticles with pDNA induced higher transgene expression in vitro for a longer duration than PEI in neural stem cells. These results suggest chitosan-TPP/HA nanoparticles may have the potential to serve as an option for gene delivery to the spinal cord.

Original language	English
Pages (from-to)	1437-1450
Number of pages	14
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	23
Issue number	11
DOIs	https://doi.org/10.1163/092050611X584090
Publication status	Published - 2012

Bibliographical note

Funding Information:
S.-J.G. and J.K.J. contributed equally to this work. This study was supported by a grant from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, Korea (Grant No. SC-4180) and the National Research Foundation in Korea funded by the Ministry of Education, Science and Technology (2010K001350).

All Science Journal Classification (ASJC) codes

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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title = "Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord",

abstract = "Gene delivery offers therapeutic promise for the treatment of neurological diseases and spinal cord injury. Several studies have offered viral vectors as vehicles to deliver therapeutic agents, yet their toxicity and immunogenicity, along with the cost of their large-scale formulation, limits their clinical use. As such, non-viral vectors are attractive in that they offer improved safety profiles compared to viruses. Poly(ethylene imine) (PEI) is one of the most extensively studied non-viral vectors, but its clinical value is limited by its cytotoxicity. Recently, chitosan/DNA complex nanoparticles have been considered as a vector for gene delivery. Here, we demonstrate that DNA nanoparticles made of hyaluronic acid (HA) and chitosan have low cytotoxicity and induce high transgene expression in neural stem cells and organotypic spinal cord slice tissue. Chitosan-TPP/HA nanoparticles were significantly less cytotoxic than PEI at various concentrations. Additionally, chitosan-TPP/HA nanoparticles with pDNA induced higher transgene expression in vitro for a longer duration than PEI in neural stem cells. These results suggest chitosan-TPP/HA nanoparticles may have the potential to serve as an option for gene delivery to the spinal cord.",

author = "Gwak, {So Jung} and Jung, {Jong Kwon} and An, {Sung Su} and Kim, {Hyo Jin} and Oh, {Jin Soo} and Pennant, {William A.} and Lee, {Hye Yeong} and Kong, {Min Ho} and Kim, {Keung Nyun} and Yoon, {Do Heum} and Yoon Ha",

note = "Funding Information: S.-J.G. and J.K.J. contributed equally to this work. This study was supported by a grant from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, Korea (Grant No. SC-4180) and the National Research Foundation in Korea funded by the Ministry of Education, Science and Technology (2010K001350).",

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AU - Ha, Yoon

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Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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