Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord

So Jung Gwak, Jong Kwon Jung, Sung Su An, Hyo Jin Kim, Jin Soo Oh, William A. Pennant, Hye Yeong Lee, Min Ho Kong, Keung Nyun Kim, Do Heum Yoon, Yoon Ha

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16 Citations (Scopus)

Abstract

Gene delivery offers therapeutic promise for the treatment of neurological diseases and spinal cord injury. Several studies have offered viral vectors as vehicles to deliver therapeutic agents, yet their toxicity and immunogenicity, along with the cost of their large-scale formulation, limits their clinical use. As such, non-viral vectors are attractive in that they offer improved safety profiles compared to viruses. Poly(ethylene imine) (PEI) is one of the most extensively studied non-viral vectors, but its clinical value is limited by its cytotoxicity. Recently, chitosan/DNA complex nanoparticles have been considered as a vector for gene delivery. Here, we demonstrate that DNA nanoparticles made of hyaluronic acid (HA) and chitosan have low cytotoxicity and induce high transgene expression in neural stem cells and organotypic spinal cord slice tissue. Chitosan-TPP/HA nanoparticles were significantly less cytotoxic than PEI at various concentrations. Additionally, chitosan-TPP/HA nanoparticles with pDNA induced higher transgene expression in vitro for a longer duration than PEI in neural stem cells. These results suggest chitosan-TPP/HA nanoparticles may have the potential to serve as an option for gene delivery to the spinal cord.

Original languageEnglish
Pages (from-to)1437-1450
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume23
Issue number11
DOIs
Publication statusPublished - 2012 Oct 12

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Gwak, S. J., Jung, J. K., An, S. S., Kim, H. J., Oh, J. S., Pennant, W. A., Lee, H. Y., Kong, M. H., Kim, K. N., Yoon, D. H., & Ha, Y. (2012). Chitosan/TPP-hyaluronic acid nanoparticles: A new vehicle for gene delivery to the spinal cord. Journal of Biomaterials Science, Polymer Edition, 23(11), 1437-1450. https://doi.org/10.1163/092050611X584090