Chlorhexidine Gluconate Attenuates the Ability of Lipoteichoic Acid from Enterococcus faecalis to Stimulate Toll-like Receptor 2

Jin Kyung Lee, Jung Eun Baik, Cheol Heui Yun, Kangseok Lee, Seung Hyun Han, Woo Cheol Lee, Kwang Shik Bae, Seung Ho Baek, Yoon Lee, Won Jun Son, Kee Yeon Kum

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Abstract

Chlorhexidine gluconate (CHX) has been widely used as a canal irrigant or an intracanal medicament on account of its antibacterial substantivity. This in vitro study aimed to determine if CHX attenuates the inflammatory activity of Enterococcus faecalis and its major virulence factor, lipoteichoic acid (LTA). An enzyme-linked immunosorbent assay showed that CHX-killed E. faecalis was less potent than heat-killed E. faecalis in the production of tumor necrosis factor α (TNF-α) by a murine macrophage cell line, RAW 264.7 (p < 0.05). Interestingly, pretreatment of LTA with 2% CHX for 6 hours or with 0.2% CHX for 24 hours almost eliminated the TNF-α inducibility (p < 0.05). Furthermore, CHX abrogated the ability of LTA to stimulate Toll-like receptor 2, resulting in the attenuated induction of TNF-α expression. Collectively, our results suggest that CHX can inactivate LTA of E. faecalis leading to the alleviation of inflammatory responses induced by E. faecalis and its LTA.

Original languageEnglish
Pages (from-to)212-215
Number of pages4
JournalJournal of Endodontics
Volume35
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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    Lee, J. K., Baik, J. E., Yun, C. H., Lee, K., Han, S. H., Lee, W. C., Bae, K. S., Baek, S. H., Lee, Y., Son, W. J., & Kum, K. Y. (2009). Chlorhexidine Gluconate Attenuates the Ability of Lipoteichoic Acid from Enterococcus faecalis to Stimulate Toll-like Receptor 2. Journal of Endodontics, 35(2), 212-215. https://doi.org/10.1016/j.joen.2008.10.018