Cholesterol biosynthesis from lanosterol

Regulation and purification of rat hepatic sterol 8-isomerase

Moon Kyu Kang, Chan Ki Kim, Tae Neung Johng, Young-Ki Paik

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The membrane bound sterol-8-isomerase (isomerase) catalyzes the anaerobic conversion of sterol-8-ene to the sterol-7-ene isomer in eucaryotes. To examine the regulatory mechanism as well as molecular characteristics of the isomerase we investigated the consequences of alteration of the enzymic activity under various diet conditions. Feeding 5% cholesterol or 0.1% AY-9944 for a minimum of 2 days caused more than a 70% decrease in microsomal isomerase activity. Feeding 5% cholestyramine plus 0.1% lovastatin (CL-diet) for 7 days led to approximately 4.0-fold induction of the isomerase activity. In addition, diurnal variation in the enzymic activity was observed with this diet. Induction of the isomerase activity by the CL-diet was quantitatively reflected in an increase in the cholesterol synthetic rate in isolated rat hepatocytes. The isomerase was highly purified from liver of rats fed the CL-diet, and its molecular mass was determined to be 21,000 Da by denaturing sodium dodecylsulfate gel electrophoresis.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalJournal of Biochemistry
Volume117
Issue number4
DOIs
Publication statusPublished - 1995 Jan 1

Fingerprint

Lanosterol
Isomerases
Biosynthesis
Purification
Rats
Nutrition
Cholesterol
Diet
Liver
Sterols
trans-1,4-Bis(2-chlorobenzaminomethyl)cyclohexane Dihydrochloride
Cholestyramine Resin
Lovastatin
Molecular mass
Electrophoresis
Isomers
delta(8)-delta(7)-sterol isomerase
Hepatocytes
Gels
Sodium

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Kang, Moon Kyu ; Kim, Chan Ki ; Johng, Tae Neung ; Paik, Young-Ki. / Cholesterol biosynthesis from lanosterol : Regulation and purification of rat hepatic sterol 8-isomerase. In: Journal of Biochemistry. 1995 ; Vol. 117, No. 4. pp. 819-823.
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Cholesterol biosynthesis from lanosterol : Regulation and purification of rat hepatic sterol 8-isomerase. / Kang, Moon Kyu; Kim, Chan Ki; Johng, Tae Neung; Paik, Young-Ki.

In: Journal of Biochemistry, Vol. 117, No. 4, 01.01.1995, p. 819-823.

Research output: Contribution to journalArticle

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