Cholesterol efflux monitoring in macrophage form cells by using fluorescence lifetime imaging

Young Sik Song, Sang Hak Lee, Byoung Hee Park, Soo Hyeok Kim, Won Sang Hwang, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Macrophages play a key role in atherosclerotic plaque destabilization and rupture, since they accumulate large amounts of lipid through the uptake of modified lipoproteins which results in foam cell formation. Cholesterol efflux is the process of removing cholesterol from macrophages in the subintima of the vessel wall, and efflux mechanism in a cell is one of the critical issues for the prevention of cardiovascular diseases. High density lipoproteins (HDL) stimulate cholesterol efflux from macrophage foam cells in the arterial wall. Radioisotope-labeled cholesterol analysis method is well known conventional method for observing cholesterol efflux. The major drawback of this method is its long and complicated process. Fluorescence intensity imaging schemes are replacing the radioisotope-labeled method in recent years for cholesterol efflux monitoring. Various spectroscopic methods are also adapted for cholesterol efflux imaging. Here we present a fluorescence lifetime imaging method for more quantitative observation of cholesterol efflux process in macrophages, which enables us to observe cholesterol level changes with various conditions. We used J774 macrophage cell and 25-NBD-cholesterol which is a famous cholesterol specific dye. Our lifetime imaging results clearly show cholesterol efflux rate very effectively. We believe that fluorescence lifetime analysis is new and very powerful for cholesterol imaging or monitoring.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
PublisherSPIE
ISBN (Electronic)9781628414189
DOIs
Publication statusPublished - 2015 Jan 1
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII - San Francisco, United States
Duration: 2015 Feb 92015 Feb 11

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9328
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII
CountryUnited States
CitySan Francisco
Period15/2/915/2/11

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Song, Y. S., Lee, S. H., Park, B. H., Kim, S. H., Hwang, W. S., & Kim, D. Y. (2015). Cholesterol efflux monitoring in macrophage form cells by using fluorescence lifetime imaging. In D. L. Farkas, D. V. Nicolau, & R. C. Leif (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII [932808] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9328). SPIE. https://doi.org/10.1117/12.2078996