CHRK1, a chitinase-related receptor-like kinase in tobacco

Youn Sung Kim, Jeong Hee Lee, Gyeong Mee Yoon, Hye Sun Cho, Seong Whan Park, Mi Chung Suh, Doil Choi, Hyun Jung Ha, Jang Ryol Liu, Hyunsook Pai

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

A cDNA encoding a chitinase-related receptor-like kinase, designated CHRK1, was isolated from tobacco(Nicotiana tabacum). The C-terminal kinase domain (KD) of CHRK1 contained all of the conserved amino acids of serine/threonine protein kinases. The putative extracellular domain was closely related to the class V chitinase of tobacco and to microbial chitinases. CHRK1 mRNA accumulation was strongly stimulated by infection with fungal pathogen and tobacco mosaic virus. Amino acid-sequence analysis revealed that the chitinase-like domain of CHRK1 lacked the essential glutamic acid residue required for chitinase activity. The recombinant chitinase-like domain did not show any catalytic activity for either oligomeric or polymeric chitin substrates. The recombinant KD of CHRK1 exhibited autophosphorylation, but the mutant KD with a mutation in the essential ATP-binding site did not, suggesting that CHRK1 encoded a functional kinase. CHRK1 was detected in membrane fractions of tobacco BY2 cells. Furthermore, CHRKI-GFP fusion protein was localized in plasma membranes when it was expressed in animal cells. This is the first report of a new type of receptor-like kinase containing a chitinase-like sequence in the putative extracellular domain.

Original languageEnglish
Pages (from-to)905-915
Number of pages11
JournalPlant physiology
Volume123
Issue number3
Publication statusPublished - 2000 Jul 1

Fingerprint

Chitinases
chitinase
Tobacco
phosphotransferases (kinases)
tobacco
Phosphotransferases
receptors
Tobacco Mosaic Virus
Chitin
Tobacco mosaic virus
Mycoses
Protein-Serine-Threonine Kinases
protein phosphorylation
Protein Sequence Analysis
chitin
glutamic acid
catalytic activity
Nicotiana tabacum
Glutamic Acid
binding sites

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Kim, Y. S., Lee, J. H., Yoon, G. M., Cho, H. S., Park, S. W., Suh, M. C., ... Pai, H. (2000). CHRK1, a chitinase-related receptor-like kinase in tobacco. Plant physiology, 123(3), 905-915.
Kim, Youn Sung ; Lee, Jeong Hee ; Yoon, Gyeong Mee ; Cho, Hye Sun ; Park, Seong Whan ; Suh, Mi Chung ; Choi, Doil ; Ha, Hyun Jung ; Liu, Jang Ryol ; Pai, Hyunsook. / CHRK1, a chitinase-related receptor-like kinase in tobacco. In: Plant physiology. 2000 ; Vol. 123, No. 3. pp. 905-915.
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abstract = "A cDNA encoding a chitinase-related receptor-like kinase, designated CHRK1, was isolated from tobacco(Nicotiana tabacum). The C-terminal kinase domain (KD) of CHRK1 contained all of the conserved amino acids of serine/threonine protein kinases. The putative extracellular domain was closely related to the class V chitinase of tobacco and to microbial chitinases. CHRK1 mRNA accumulation was strongly stimulated by infection with fungal pathogen and tobacco mosaic virus. Amino acid-sequence analysis revealed that the chitinase-like domain of CHRK1 lacked the essential glutamic acid residue required for chitinase activity. The recombinant chitinase-like domain did not show any catalytic activity for either oligomeric or polymeric chitin substrates. The recombinant KD of CHRK1 exhibited autophosphorylation, but the mutant KD with a mutation in the essential ATP-binding site did not, suggesting that CHRK1 encoded a functional kinase. CHRK1 was detected in membrane fractions of tobacco BY2 cells. Furthermore, CHRKI-GFP fusion protein was localized in plasma membranes when it was expressed in animal cells. This is the first report of a new type of receptor-like kinase containing a chitinase-like sequence in the putative extracellular domain.",
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Kim, YS, Lee, JH, Yoon, GM, Cho, HS, Park, SW, Suh, MC, Choi, D, Ha, HJ, Liu, JR & Pai, H 2000, 'CHRK1, a chitinase-related receptor-like kinase in tobacco', Plant physiology, vol. 123, no. 3, pp. 905-915.

CHRK1, a chitinase-related receptor-like kinase in tobacco. / Kim, Youn Sung; Lee, Jeong Hee; Yoon, Gyeong Mee; Cho, Hye Sun; Park, Seong Whan; Suh, Mi Chung; Choi, Doil; Ha, Hyun Jung; Liu, Jang Ryol; Pai, Hyunsook.

In: Plant physiology, Vol. 123, No. 3, 01.07.2000, p. 905-915.

Research output: Contribution to journalArticle

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Kim YS, Lee JH, Yoon GM, Cho HS, Park SW, Suh MC et al. CHRK1, a chitinase-related receptor-like kinase in tobacco. Plant physiology. 2000 Jul 1;123(3):905-915.