CHRK1, a chitinase-related receptor-like kinase, plays a role in plant development and cytokinin homeostasis in tobacco

Jeong Hee Lee, Kentaro Takei, Hitoshi Sakakibara, Hye Sun Cho, Do Myung Kim, Youn Sung Kim, Sung Ran Min, Woo Taek Kim, Dae Young Sohn, Yong Pyo Lim, Hyunsook Pai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

CHRK1 encodes a receptor-like kinase that contains a chitinase-related sequence in the extracellular domain in Nicotiana tabacum. In this study, we showed that CHRK1 is mainly expressed in the shoot apex region including leaf primordia and young leaves, and germinating seedlings and vascular tissues, based on GUS activity of transgenic tobacco plants carrying the CHRK1 promoter-GUS fusion gene. Transgenic tobacco plants in which CHRK1 expression was suppressed exhibited pleiotrophic developmental abnormality, including formation of proliferating shooty calli from emerging seedlings and severely altered seedling development. At the cellular level, ectopic cell proliferation, reduced cell specificity, and aberrant chloroplast development were observed. The transgenic lines contained 3-fold higher level of cytokinin than the wild-type plants. Consistently, the transgenic seedlings exhibited a typical cytokinin response in the absence of hormone, such as deetiolation under the dark. Based on these results, we propose that CHRK1 is involved in a developmental signaling pathway regulating cell proliferation/differentiation and the endogenous cytokinin levels in tobacco.

Original languageEnglish
Pages (from-to)877-890
Number of pages14
JournalPlant Molecular Biology
Volume53
Issue number6
DOIs
Publication statusPublished - 2003 Dec 1

Fingerprint

Cytokinins
Chitinases
Plant Development
chitinase
Seedlings
cytokinins
Tobacco
plant development
homeostasis
phosphotransferases (kinases)
Homeostasis
tobacco
Phosphotransferases
genetically modified organisms
receptors
seedlings
Genetically Modified Plants
Etiolation
cell proliferation
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Lee, Jeong Hee ; Takei, Kentaro ; Sakakibara, Hitoshi ; Cho, Hye Sun ; Kim, Do Myung ; Kim, Youn Sung ; Min, Sung Ran ; Kim, Woo Taek ; Sohn, Dae Young ; Lim, Yong Pyo ; Pai, Hyunsook. / CHRK1, a chitinase-related receptor-like kinase, plays a role in plant development and cytokinin homeostasis in tobacco. In: Plant Molecular Biology. 2003 ; Vol. 53, No. 6. pp. 877-890.
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CHRK1, a chitinase-related receptor-like kinase, plays a role in plant development and cytokinin homeostasis in tobacco. / Lee, Jeong Hee; Takei, Kentaro; Sakakibara, Hitoshi; Cho, Hye Sun; Kim, Do Myung; Kim, Youn Sung; Min, Sung Ran; Kim, Woo Taek; Sohn, Dae Young; Lim, Yong Pyo; Pai, Hyunsook.

In: Plant Molecular Biology, Vol. 53, No. 6, 01.12.2003, p. 877-890.

Research output: Contribution to journalArticle

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AU - Min, Sung Ran

AU - Kim, Woo Taek

AU - Sohn, Dae Young

AU - Lim, Yong Pyo

AU - Pai, Hyunsook

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