Chromatin remodeling complex interacts with ADD1/SREBP1c to mediate insulin-dependent regulation of gene expression

Yun Sok Lee, Dong Hyun Sohn, Daehee Han, Han Woong Lee, Rho Hyun Seong, Jae Bum Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Insulin plays a critical role in whole-body energy homeostasis by regulating lipid and glucose metabolism. In fat and liver tissues, ADD1/SREBP1c is a key transcription factor to mediate insulin-dependent regulation of gene expression. Although transcriptional and proteolytic activation of ADD1/SREBP1c has been studied intensively, the mechanism by which insulin regulates expression of its target genes with ADD1/SREBP1c at the chromatin level is unclear. Here, we reveal that SWI/SNF chromatin remodeling factors interact with the ADD1/SREBP1c and actively regulate insulin-dependent gene expression. Insulin enhanced recruitment of SWI/SNF chromatin remodeling factors to its target gene promoters with concomitant changes in the chromatin structures as well as gene expression. Furthermore, in vivo overexpression of BAF155/SRG3, a component of the SWI/SNF complex, substantially promoted insulin target gene expression and insulin sensitivity. Taken together, our results suggest that the SWI/SNF chromatin remodeling complexes confer not only insulin-dependent gene expression but also insulin sensitivity in vivo via interaction with ADD1/SREBP1c.

Original languageEnglish
Pages (from-to)438-452
Number of pages15
JournalMolecular and Cellular Biology
Volume27
Issue number2
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Chromatin Assembly and Disassembly
Gene Expression Regulation
Insulin
Gene Expression
Chromatin
Insulin Resistance
Lipid Metabolism
Transcriptional Activation
Genes
Homeostasis
Transcription Factors
Fats
Glucose
Liver

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Lee, Yun Sok ; Sohn, Dong Hyun ; Han, Daehee ; Lee, Han Woong ; Seong, Rho Hyun ; Kim, Jae Bum. / Chromatin remodeling complex interacts with ADD1/SREBP1c to mediate insulin-dependent regulation of gene expression. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 2. pp. 438-452.
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Chromatin remodeling complex interacts with ADD1/SREBP1c to mediate insulin-dependent regulation of gene expression. / Lee, Yun Sok; Sohn, Dong Hyun; Han, Daehee; Lee, Han Woong; Seong, Rho Hyun; Kim, Jae Bum.

In: Molecular and Cellular Biology, Vol. 27, No. 2, 01.01.2007, p. 438-452.

Research output: Contribution to journalArticle

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