TY - JOUR
T1 - Chromosome-encoded AmpC and CTX-M extended-spectrum β-lactamases in clinical isolates of Proteus mirabilis from Korea
AU - Song, Wonkeun
AU - Kim, Juwon
AU - Bae, Il Kwon
AU - Jeong, Seok Hoon
AU - Seo, Young Hee
AU - Shin, Jong Hee
AU - Jang, Sook Jin
AU - Uh, Young
AU - Shin, Jeong Hwan
AU - Lee, Mi Kyung
AU - Lee, Kyungwon
PY - 2011/4
Y1 - 2011/4
N2 - Among 222 Proteus mirabilis clinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments was bla CTX-M-14a (n = 12). The blaCTX-M-90 (n = 4), bla CTX-M-15 (n = 3), blaCTX-M-12 (n = 3), bla CTX-M-2 (n = 2), blaCTX-M-14b (n = 1), bla TEM-52 (n = 5), and blaSHV-12 (n = 1) genes were also detected. Eight isolates carried an AmpC β-lactamase gene, such as bla CMY-2 (n = 6) or blaDHA-1 (n = 2). All bla genes encoding CTX-M-1- and CTX-M-9-type enzymes and all blaCMY-2 genes were preceded by ISEcp1-like elements. The blaCTX-M-2 gene found in two isolates was located on a complex class 1 integron. The blaDHA-1 gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. The blaCTX-M genes were located on the chromosome in 21 isolates. A plasmid location for the blaCTX-M gene was found in only four isolates: the blaCTX-M-14a gene was located on ∼150-kbp IncA/C plasmids in three isolates and on a ∼50-kbp IncN plasmid in one isolate. The blaTEM-52 gene was located on ∼50-kbp IncN plasmids in all five isolates. The AmpC β-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried the blaCMY-2 gene on a ∼150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC β-lactamase genes in P. mirabilis is no longer an unusual phenomenon in hospital environments.
AB - Among 222 Proteus mirabilis clinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments was bla CTX-M-14a (n = 12). The blaCTX-M-90 (n = 4), bla CTX-M-15 (n = 3), blaCTX-M-12 (n = 3), bla CTX-M-2 (n = 2), blaCTX-M-14b (n = 1), bla TEM-52 (n = 5), and blaSHV-12 (n = 1) genes were also detected. Eight isolates carried an AmpC β-lactamase gene, such as bla CMY-2 (n = 6) or blaDHA-1 (n = 2). All bla genes encoding CTX-M-1- and CTX-M-9-type enzymes and all blaCMY-2 genes were preceded by ISEcp1-like elements. The blaCTX-M-2 gene found in two isolates was located on a complex class 1 integron. The blaDHA-1 gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. The blaCTX-M genes were located on the chromosome in 21 isolates. A plasmid location for the blaCTX-M gene was found in only four isolates: the blaCTX-M-14a gene was located on ∼150-kbp IncA/C plasmids in three isolates and on a ∼50-kbp IncN plasmid in one isolate. The blaTEM-52 gene was located on ∼50-kbp IncN plasmids in all five isolates. The AmpC β-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried the blaCMY-2 gene on a ∼150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC β-lactamase genes in P. mirabilis is no longer an unusual phenomenon in hospital environments.
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U2 - 10.1128/AAC.01835-09
DO - 10.1128/AAC.01835-09
M3 - Article
C2 - 21282448
AN - SCOPUS:79953188714
VL - 55
SP - 1414
EP - 1419
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 4
ER -
