Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome

Giovanna Oggé, Roberto Romero, Deug Chan Lee, Francesca Gotsch, Nandor Gabor Than, Joonho Lee, Tinnakorn Chaiworapongsa, Zhong Dong, Pooja Mittal, Sonia S. Hassan, Chong Jai Kim

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Acute chorioamnionitis of infectious origin and chronic chorioamnionitis of immunological origin are two major placental lesions of spontaneous preterm birth with elevated amniotic fluid interleukin-6 and CXCL10 concentrations, respectively. The changes in the amniotic fluid proteome associated with intra-amniotic infection and acute chorioamnionitis are well defined, yet alterations unique to chronic chorioamnionitis remain to be elucidated. This study was conducted to determine those amniotic fluid proteins changing specifically in the presence of chronic chorioamnionitis. Amniotic fluid obtained from acute chorioamnionitis, chronic chorioamnionitis and gestational age-matched controls were analysed by two-dimensional (2D) difference in gel electrophoresis and MALDI-TOF analyses. The type of histological inflammation was used to define each condition in preterm labour cases (n = 125) and term not in labour cases (n = 22), and the amniotic fluid concentrations of interleukin-6, CXCL8, CXCL10 and prostaglandin F were also measured by specific immunoassays. Among preterm labour cases, 31 differentially expressed proteins were identified in chronic chorioamnionitis cases as compared to both acute chorioamnionitis and control cases. Importantly, glycodelin-A, which maintains maternal tolerance against an allogeneic fetus, was decreased in chronic chorioamnionitis, while haptoglobin was increased. We report the amniotic fluid proteome of chronic chorioamnionitis for the first time, and the findings herein strongly suggest that there is a pathophysiological association between the changes of immunomodulatory proteins in the amniotic fluid and chronic chorioamnionitis, a histological manifestation of maternal anti-fetal allograft rejection.

Original languageEnglish
Pages (from-to)553-565
Number of pages13
JournalJournal of Pathology
Volume223
Issue number4
DOIs
Publication statusPublished - 2011 Mar 1

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Chorioamnionitis
Amniotic Fluid
Proteome
Premature Obstetric Labor
Interleukin-6
Mothers
Proteins
Haptoglobins
Dinoprost
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Premature Birth
Immunoassay
Gestational Age
Allografts
Electrophoresis
Fetus

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Oggé, G., Romero, R., Lee, D. C., Gotsch, F., Than, N. G., Lee, J., ... Kim, C. J. (2011). Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome. Journal of Pathology, 223(4), 553-565. https://doi.org/10.1002/path.2825
Oggé, Giovanna ; Romero, Roberto ; Lee, Deug Chan ; Gotsch, Francesca ; Than, Nandor Gabor ; Lee, Joonho ; Chaiworapongsa, Tinnakorn ; Dong, Zhong ; Mittal, Pooja ; Hassan, Sonia S. ; Kim, Chong Jai. / Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome. In: Journal of Pathology. 2011 ; Vol. 223, No. 4. pp. 553-565.
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Oggé, G, Romero, R, Lee, DC, Gotsch, F, Than, NG, Lee, J, Chaiworapongsa, T, Dong, Z, Mittal, P, Hassan, SS & Kim, CJ 2011, 'Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome', Journal of Pathology, vol. 223, no. 4, pp. 553-565. https://doi.org/10.1002/path.2825

Chronic chorioamnionitis displays distinct alterations of the amniotic fluid proteome. / Oggé, Giovanna; Romero, Roberto; Lee, Deug Chan; Gotsch, Francesca; Than, Nandor Gabor; Lee, Joonho; Chaiworapongsa, Tinnakorn; Dong, Zhong; Mittal, Pooja; Hassan, Sonia S.; Kim, Chong Jai.

In: Journal of Pathology, Vol. 223, No. 4, 01.03.2011, p. 553-565.

Research output: Contribution to journalArticle

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AU - Oggé, Giovanna

AU - Romero, Roberto

AU - Lee, Deug Chan

AU - Gotsch, Francesca

AU - Than, Nandor Gabor

AU - Lee, Joonho

AU - Chaiworapongsa, Tinnakorn

AU - Dong, Zhong

AU - Mittal, Pooja

AU - Hassan, Sonia S.

AU - Kim, Chong Jai

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