Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia

In Koo Hwang, Seon Gil Do, Ki Yeon Yoo, Duk Soo Kim, Jun Hwi Cho, Young-Guen Kwon, Jae Yong Lee, Yang Seok Oh, Tae Cheon Kang, Moo Ho Won

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyrus. NF-150 immunoreactivity and protein contents of NF-150 and RT 97 (polyphosphorylation epitopes of neurofilament) were significantly decreased at 15 min after ischemic insult. Between 30 min and 12 h after ischemic insult, NF-150 immunoreactivity and protein content were significantly increased as compared with the sham-operated group. Thereafter, NF-150 immunoreactivity and protein content started to decrease. At 12 h after ischemic insult, unlike dentate gyrus, NF-150 immunoreactivity increased in pyramidal cells of the CA1 region. Thereafter, NF-150 immunoreactivity in the CA1 region started to decrease, and 4 days after ischemic insult, NF-150 immunoreactivity nearly was similar to that of the sham-operated group. These biphasic patterns of NF-150 immunoreactivity in the hippocampus and dentate gyrus are reverse correlated with that of the intracellular calcium influx. For calcium detection in the CA1 region, we also conducted alizarin red staining. Alizarin red positive neurons were detected in some neurons at 15-30 min after ischemic insult. At 12 h after ischemia, alizarin red positive neurons were decreased. Thereafter, alizarin red positive neurons started to decrease, but alizarin positive neurons were significantly increased in dying neurons 4 days after ischemia. These results suggest that ischemia-related changes of NF-150 expression may be caused by the calcium following transient forebrain ischemia.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalBrain Research
Volume1016
Issue number1
DOIs
Publication statusPublished - 2004 Jul 30

Fingerprint

Intermediate Filaments
Gerbillinae
Dentate Gyrus
Prosencephalon
Hippocampus
Ischemia
Neurons
Calcium
Pyramidal Cells
Epitopes
alizarin
Staining and Labeling
neurofilament protein 150

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Hwang, In Koo ; Do, Seon Gil ; Yoo, Ki Yeon ; Kim, Duk Soo ; Cho, Jun Hwi ; Kwon, Young-Guen ; Lee, Jae Yong ; Oh, Yang Seok ; Kang, Tae Cheon ; Won, Moo Ho. / Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia. In: Brain Research. 2004 ; Vol. 1016, No. 1. pp. 119-128.
@article{84b19265799f48df9b0ae4da52fc0462,
title = "Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia",
abstract = "In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyrus. NF-150 immunoreactivity and protein contents of NF-150 and RT 97 (polyphosphorylation epitopes of neurofilament) were significantly decreased at 15 min after ischemic insult. Between 30 min and 12 h after ischemic insult, NF-150 immunoreactivity and protein content were significantly increased as compared with the sham-operated group. Thereafter, NF-150 immunoreactivity and protein content started to decrease. At 12 h after ischemic insult, unlike dentate gyrus, NF-150 immunoreactivity increased in pyramidal cells of the CA1 region. Thereafter, NF-150 immunoreactivity in the CA1 region started to decrease, and 4 days after ischemic insult, NF-150 immunoreactivity nearly was similar to that of the sham-operated group. These biphasic patterns of NF-150 immunoreactivity in the hippocampus and dentate gyrus are reverse correlated with that of the intracellular calcium influx. For calcium detection in the CA1 region, we also conducted alizarin red staining. Alizarin red positive neurons were detected in some neurons at 15-30 min after ischemic insult. At 12 h after ischemia, alizarin red positive neurons were decreased. Thereafter, alizarin red positive neurons started to decrease, but alizarin positive neurons were significantly increased in dying neurons 4 days after ischemia. These results suggest that ischemia-related changes of NF-150 expression may be caused by the calcium following transient forebrain ischemia.",
author = "Hwang, {In Koo} and Do, {Seon Gil} and Yoo, {Ki Yeon} and Kim, {Duk Soo} and Cho, {Jun Hwi} and Young-Guen Kwon and Lee, {Jae Yong} and Oh, {Yang Seok} and Kang, {Tae Cheon} and Won, {Moo Ho}",
year = "2004",
month = "7",
day = "30",
doi = "10.1016/j.brainres.2004.05.001",
language = "English",
volume = "1016",
pages = "119--128",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia. / Hwang, In Koo; Do, Seon Gil; Yoo, Ki Yeon; Kim, Duk Soo; Cho, Jun Hwi; Kwon, Young-Guen; Lee, Jae Yong; Oh, Yang Seok; Kang, Tae Cheon; Won, Moo Ho.

In: Brain Research, Vol. 1016, No. 1, 30.07.2004, p. 119-128.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia

AU - Hwang, In Koo

AU - Do, Seon Gil

AU - Yoo, Ki Yeon

AU - Kim, Duk Soo

AU - Cho, Jun Hwi

AU - Kwon, Young-Guen

AU - Lee, Jae Yong

AU - Oh, Yang Seok

AU - Kang, Tae Cheon

AU - Won, Moo Ho

PY - 2004/7/30

Y1 - 2004/7/30

N2 - In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyrus. NF-150 immunoreactivity and protein contents of NF-150 and RT 97 (polyphosphorylation epitopes of neurofilament) were significantly decreased at 15 min after ischemic insult. Between 30 min and 12 h after ischemic insult, NF-150 immunoreactivity and protein content were significantly increased as compared with the sham-operated group. Thereafter, NF-150 immunoreactivity and protein content started to decrease. At 12 h after ischemic insult, unlike dentate gyrus, NF-150 immunoreactivity increased in pyramidal cells of the CA1 region. Thereafter, NF-150 immunoreactivity in the CA1 region started to decrease, and 4 days after ischemic insult, NF-150 immunoreactivity nearly was similar to that of the sham-operated group. These biphasic patterns of NF-150 immunoreactivity in the hippocampus and dentate gyrus are reverse correlated with that of the intracellular calcium influx. For calcium detection in the CA1 region, we also conducted alizarin red staining. Alizarin red positive neurons were detected in some neurons at 15-30 min after ischemic insult. At 12 h after ischemia, alizarin red positive neurons were decreased. Thereafter, alizarin red positive neurons started to decrease, but alizarin positive neurons were significantly increased in dying neurons 4 days after ischemia. These results suggest that ischemia-related changes of NF-150 expression may be caused by the calcium following transient forebrain ischemia.

AB - In this study, we observed the chronological alterations of neurofilament 150 (NF-150) immunoreactivity in the gerbil hippocampus and dentate gyrus after 5 min transient forebrain ischemia. NF-150 immunoreactivity in the sham-operated group was mainly detected in mossy fibers and in the hilar region of the dentate gyrus. NF-150 immunoreactivity and protein contents of NF-150 and RT 97 (polyphosphorylation epitopes of neurofilament) were significantly decreased at 15 min after ischemic insult. Between 30 min and 12 h after ischemic insult, NF-150 immunoreactivity and protein content were significantly increased as compared with the sham-operated group. Thereafter, NF-150 immunoreactivity and protein content started to decrease. At 12 h after ischemic insult, unlike dentate gyrus, NF-150 immunoreactivity increased in pyramidal cells of the CA1 region. Thereafter, NF-150 immunoreactivity in the CA1 region started to decrease, and 4 days after ischemic insult, NF-150 immunoreactivity nearly was similar to that of the sham-operated group. These biphasic patterns of NF-150 immunoreactivity in the hippocampus and dentate gyrus are reverse correlated with that of the intracellular calcium influx. For calcium detection in the CA1 region, we also conducted alizarin red staining. Alizarin red positive neurons were detected in some neurons at 15-30 min after ischemic insult. At 12 h after ischemia, alizarin red positive neurons were decreased. Thereafter, alizarin red positive neurons started to decrease, but alizarin positive neurons were significantly increased in dying neurons 4 days after ischemia. These results suggest that ischemia-related changes of NF-150 expression may be caused by the calcium following transient forebrain ischemia.

UR - http://www.scopus.com/inward/record.url?scp=3042611516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042611516&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2004.05.001

DO - 10.1016/j.brainres.2004.05.001

M3 - Article

VL - 1016

SP - 119

EP - 128

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -