Circularization of an RNA template via long-range base pairing is critical for hepadnaviral reverse transcription

Myeong Kyun Shin, Jong Hoon Kim, Dong Kyun Ryu, Wang Shick Ryu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Although an overall genetic strategy for hepadnaviral reverse transcription has been established, the mechanism that underlies the minus-strand transfer is still poorly defined. We and others independently identified a novel cis-acting element, termed β or φ{symbol}, respectively, that is critical for the minus-strand DNA synthesis of hepatitis B virus. A 5′-3′, long-range interaction of the RNA template was proposed that involves the 5′ ε sequence (encapsidation signal) and the 3′ β/φ{symbol} sequence. We subjected the hypothesized base pairing to genetic analysis. The data indicated that mutations abrogating the hypothesized base pairing markedly impaired minus-strand DNA synthesis, while compensatory mutations that restored the base pairing rescued the minus-strand DNA synthesis. These results demonstrated the critical role of the 5′-3′, long-range interaction in minus-strand DNA synthesis. We speculate that such a long-range interaction may precisely juxtapose a donor to an acceptor during minus-strand transfer.

Original languageEnglish
Pages (from-to)362-373
Number of pages12
JournalVirology
Volume371
Issue number2
DOIs
Publication statusPublished - 2008 Feb 20

Bibliographical note

Funding Information:
We thank Drs. Byung-Yoon Ahn (Korea University) and Jehan Lee (Yonsei University) for their critical reading of this manuscript. This work was supported by the Korean Research Foundation Grant (MOEHRD) (KRF-2004-041-C00286).

All Science Journal Classification (ASJC) codes

  • Virology

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