Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status)

Jean Kyung Paik, Ji Young Kim, Oh Yoen Kim, Yonghee Lee, Tae Sook Jeong, Gary Sweeney, Yangsoo Jang, Jong Ho Lee

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Abstract

Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.

Original languageEnglish
Article numbere29675
JournalPLoS One
Volume7
Issue number2
DOIs
Publication statusPublished - 2012 Feb 16

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1-Alkyl-2-acetylglycerophosphocholine Esterase
menopause
Women's Rights
Oxidative stress
mononuclear leukocytes
lipoproteins
Blood Cells
oxen
Oxidative Stress
Blood
oxidative stress
inflammation
Inflammation
Plasmas
interleukin-6
Lipoproteins
tumor necrosis factors
Interleukin-6
interleukin-1
Interleukin-1

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Paik, Jean Kyung ; Kim, Ji Young ; Kim, Oh Yoen ; Lee, Yonghee ; Jeong, Tae Sook ; Sweeney, Gary ; Jang, Yangsoo ; Lee, Jong Ho. / Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status). In: PLoS One. 2012 ; Vol. 7, No. 2.
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title = "Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status)",
abstract = "Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.",
author = "Paik, {Jean Kyung} and Kim, {Ji Young} and Kim, {Oh Yoen} and Yonghee Lee and Jeong, {Tae Sook} and Gary Sweeney and Yangsoo Jang and Lee, {Jong Ho}",
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Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status). / Paik, Jean Kyung; Kim, Ji Young; Kim, Oh Yoen; Lee, Yonghee; Jeong, Tae Sook; Sweeney, Gary; Jang, Yangsoo; Lee, Jong Ho.

In: PLoS One, Vol. 7, No. 2, e29675, 16.02.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status)

AU - Paik, Jean Kyung

AU - Kim, Ji Young

AU - Kim, Oh Yoen

AU - Lee, Yonghee

AU - Jeong, Tae Sook

AU - Sweeney, Gary

AU - Jang, Yangsoo

AU - Lee, Jong Ho

PY - 2012/2/16

Y1 - 2012/2/16

N2 - Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.

AB - Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.

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U2 - 10.1371/journal.pone.0029675

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