Abstract
Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.
| Original language | English |
|---|---|
| Article number | e29675 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Feb 16 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
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Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status). / Paik, Jean Kyung; Kim, Ji Young; Kim, Oh Yoen; Lee, Yonghee; Jeong, Tae Sook; Sweeney, Gary; Jang, Yangsoo; Lee, Jong Ho.
In: PLoS One, Vol. 7, No. 2, e29675, 16.02.2012.Research output: Contribution to journal › Article
TY - JOUR
T1 - Circulating and PBMC Lp-PLA 2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status)
AU - Paik, Jean Kyung
AU - Kim, Ji Young
AU - Kim, Oh Yoen
AU - Lee, Yonghee
AU - Jeong, Tae Sook
AU - Sweeney, Gary
AU - Jang, Yangsoo
AU - Lee, Jong Ho
PY - 2012/2/16
Y1 - 2012/2/16
N2 - Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.
AB - Background: This study aimed to determine the association of lipoprotein-associated phospholipase A 2 (Lp-PLA 2) activity in circulation and peripheral blood mononuclear cells (PBMCs) with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA 2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. Methodology/Principal Findings: Eighty postmenopausal women (53.0±4.05 yr) and 96 premenopausal women (39.7±9.25 yr) participated in this study. Lp-PLA 2 activities, interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA 2 activity positively correlated with Lp-PLA 2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA 2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L), a significant increase in Lp-PLA 2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA 2 activity positively correlated with unstimulated PBMC Lp-PLA 2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001), whereas in the high ox-LDL circulating Lp-PLA 2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014) but not with Lp-PLA 2 activity in PBMCs. Conclusions/Significance: The lack of relation between circulating Lp-PLA 2 activity and Lp-PLA 2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA 2 activity except PBMC in postmenopausal women with high ox-LDL. We also demonstrated that circulating Lp-PLA 2 and PBMC secreted Lp-PLA 2 associate differently with markers of oxidative stress and sub clinical inflammation in nonobese women, particularly according to the menopausal states.
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U2 - 10.1371/journal.pone.0029675
DO - 10.1371/journal.pone.0029675
M3 - Article
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 2
M1 - e29675
ER -