With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. T his process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. H ere we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brainmapping studies.
Bibliographical noteFunding Information:
We acknowledge all members of the Deisseroth laboratory for discussions and support. This work was funded by a US National Institutes of Health Director’s Transformative Research Award (TR01) to K.D. from the National Institute of Mental Health, as well as by the National Science Foundation, the Simons Foundation, the President and Provost of Stanford University, and the Howard Hughes Medical Institute. K.D. is also funded by the National Institute on Drug Abuse and the Defense Advanced Research Projects Agency Reorganization and Plasticity to Accelerate Injury Recovery program, and the Wiegers, Snyder, Reeves, Gatsby, and Yu Foundations. K.C. is supported by the Burroughs Wellcome Fund Career Award at the Scientific Interface.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology