Clinical significance of microsatellite instability in sporadic epithelial ovarian tumors

Bo Sung Yoon, Young Tae Kim, Jae Hoon Kim, Sang Wun Kim, Eun Ji Nam, Nam Hoon Cho, Jae Wook Kim, Sunghoon Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: We evaluated the expression of microsatellite instability (MSI) in sporadic ovarian tumors using 5 standard and 9 new MSI markers to determine the clinical significance of MSI in sporadic epithelial ovarian tumors. Materials and Methods: MSI was examined in 21 borderline and 25 malignant ovarian tumors. Polymerase chain reaction (PCR) was performed using the 5 markers recommended by the National Cancer Institute (NCI) for colon cancer and 9 additional markers. MSI was determined using fractional analysis by mixing the PCR products and size markers. Results: Using the 5 conventional MSI markers, MSI was found in 4 of 46 (8.6%) ovarian tumors, including 2 of 21 (9.51/6) borderline ovarian tumors and 2 of 25 (8%) malignant ovarian tumors. Using the 9 additional MSI markers, MSI was observed in 7 of 46 (15.2%) ovarian tumors, including 3 of 21 (14.3%) borderline ovarian tumors and 4 of 25 (16%) malignant ovarian tumors. There was no statistically significant difference between MSI and clinicopathological factors, including histology and stage, although there was a trend toward an increased incidence of MSI in the serous type. Conclusion: MSI was infrequent in ovarian tumors, including both borderline and malignant tumors. MSI was found to be uncommon in sporadic ovarian tumors, even by using additional MSI markers. The clinical significance of MSI is not strong in patients with sporadic ovarian tumors.

Original languageEnglish
Pages (from-to)272-278
Number of pages7
JournalYonsei medical journal
Volume49
Issue number2
DOIs
Publication statusPublished - 2008 Apr 1

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Microsatellite Instability
Neoplasms
Microsatellite Repeats
Colonic Neoplasms
Polymerase Chain Reaction
National Cancer Institute (U.S.)

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Yoon, Bo Sung ; Kim, Young Tae ; Kim, Jae Hoon ; Kim, Sang Wun ; Nam, Eun Ji ; Cho, Nam Hoon ; Kim, Jae Wook ; Kim, Sunghoon. / Clinical significance of microsatellite instability in sporadic epithelial ovarian tumors. In: Yonsei medical journal. 2008 ; Vol. 49, No. 2. pp. 272-278.
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abstract = "Purpose: We evaluated the expression of microsatellite instability (MSI) in sporadic ovarian tumors using 5 standard and 9 new MSI markers to determine the clinical significance of MSI in sporadic epithelial ovarian tumors. Materials and Methods: MSI was examined in 21 borderline and 25 malignant ovarian tumors. Polymerase chain reaction (PCR) was performed using the 5 markers recommended by the National Cancer Institute (NCI) for colon cancer and 9 additional markers. MSI was determined using fractional analysis by mixing the PCR products and size markers. Results: Using the 5 conventional MSI markers, MSI was found in 4 of 46 (8.6{\%}) ovarian tumors, including 2 of 21 (9.51/6) borderline ovarian tumors and 2 of 25 (8{\%}) malignant ovarian tumors. Using the 9 additional MSI markers, MSI was observed in 7 of 46 (15.2{\%}) ovarian tumors, including 3 of 21 (14.3{\%}) borderline ovarian tumors and 4 of 25 (16{\%}) malignant ovarian tumors. There was no statistically significant difference between MSI and clinicopathological factors, including histology and stage, although there was a trend toward an increased incidence of MSI in the serous type. Conclusion: MSI was infrequent in ovarian tumors, including both borderline and malignant tumors. MSI was found to be uncommon in sporadic ovarian tumors, even by using additional MSI markers. The clinical significance of MSI is not strong in patients with sporadic ovarian tumors.",
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Clinical significance of microsatellite instability in sporadic epithelial ovarian tumors. / Yoon, Bo Sung; Kim, Young Tae; Kim, Jae Hoon; Kim, Sang Wun; Nam, Eun Ji; Cho, Nam Hoon; Kim, Jae Wook; Kim, Sunghoon.

In: Yonsei medical journal, Vol. 49, No. 2, 01.04.2008, p. 272-278.

Research output: Contribution to journalArticle

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N2 - Purpose: We evaluated the expression of microsatellite instability (MSI) in sporadic ovarian tumors using 5 standard and 9 new MSI markers to determine the clinical significance of MSI in sporadic epithelial ovarian tumors. Materials and Methods: MSI was examined in 21 borderline and 25 malignant ovarian tumors. Polymerase chain reaction (PCR) was performed using the 5 markers recommended by the National Cancer Institute (NCI) for colon cancer and 9 additional markers. MSI was determined using fractional analysis by mixing the PCR products and size markers. Results: Using the 5 conventional MSI markers, MSI was found in 4 of 46 (8.6%) ovarian tumors, including 2 of 21 (9.51/6) borderline ovarian tumors and 2 of 25 (8%) malignant ovarian tumors. Using the 9 additional MSI markers, MSI was observed in 7 of 46 (15.2%) ovarian tumors, including 3 of 21 (14.3%) borderline ovarian tumors and 4 of 25 (16%) malignant ovarian tumors. There was no statistically significant difference between MSI and clinicopathological factors, including histology and stage, although there was a trend toward an increased incidence of MSI in the serous type. Conclusion: MSI was infrequent in ovarian tumors, including both borderline and malignant tumors. MSI was found to be uncommon in sporadic ovarian tumors, even by using additional MSI markers. The clinical significance of MSI is not strong in patients with sporadic ovarian tumors.

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