Clinicopathological and molecular characteristics of mammary adenoid cystic carcinoma with adipocytic differentiation with emphasis on the identification of a novel BRAF mutation

Go Eun Bae, Nara Yoon, Eun Yoon Cho, Hyun Soo Kim, Soo Youn Cho

Research output: Contribution to journalArticle

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Abstract

Background/Aim: Adenoid cystic carcinoma accounts for fewer than 1% of all breast malignancies. Herein, we describe the clinicopathological characteristics and immunophenotype of mammary adenoid cystic carcinoma that exhibited adipocytic differentiation. Furthermore, we aimed to identify mutations potentially associated with this rare tumor. Case Report: A 52-year-old woman was referred to our Institution after detection of an incidental breast mass. The resected breast specimen contained a well-circumscribed, firm mass surrounded by ill-defined, soft, yellow bulging nodules and measured 2.5 cm in the greatest dimension. The surgically-resected breast lesion was subjected to pathological examination and immunohistochemical staining. Next-generation sequencing was also carried out on an Ion Torrent Personal Genome Machine, using Ion AmpliSeq Cancer Hotspot Panel v2. Histologically, the tumor had two components: a central area of complex glandular proliferation with solid and cribriform architectures and a peripheral area showing adipocytic differentiation with spindle cell proliferation. Immunohistochemical staining revealed positive immunoreactivity for cytokeratin 5/6, epidermal growth factor receptor, and smooth muscle actin in both the central and peripheral areas. Next-generation sequencing-based genetic analysis identified a homozygous missense mutation (NM_004333.4:c.1742A>G; p.N581S) in the v-Raf murine sarcoma viral oncogene homolog B (BRAF) gene. Conclusion: We report the first case, as far as we are aware, of primary mammary adenoid cystic carcinoma with adipocytic differentiation. A novel missense mutation in BRAF was detected. The potential response to BRAF inhibitors in the presence of this mutation remains a matter for further studies.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalAnticancer research
Volume39
Issue number1
DOIs
Publication statusPublished - 2019 Jan

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Adenoid Cystic Carcinoma
Breast
Mutation
Missense Mutation
Neoplasms
Keratin-6
Keratin-5
Ions
Staining and Labeling
Oncogenes
Epidermal Growth Factor Receptor
Sarcoma
Smooth Muscle
Actins
Cell Proliferation
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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title = "Clinicopathological and molecular characteristics of mammary adenoid cystic carcinoma with adipocytic differentiation with emphasis on the identification of a novel BRAF mutation",
abstract = "Background/Aim: Adenoid cystic carcinoma accounts for fewer than 1{\%} of all breast malignancies. Herein, we describe the clinicopathological characteristics and immunophenotype of mammary adenoid cystic carcinoma that exhibited adipocytic differentiation. Furthermore, we aimed to identify mutations potentially associated with this rare tumor. Case Report: A 52-year-old woman was referred to our Institution after detection of an incidental breast mass. The resected breast specimen contained a well-circumscribed, firm mass surrounded by ill-defined, soft, yellow bulging nodules and measured 2.5 cm in the greatest dimension. The surgically-resected breast lesion was subjected to pathological examination and immunohistochemical staining. Next-generation sequencing was also carried out on an Ion Torrent Personal Genome Machine, using Ion AmpliSeq Cancer Hotspot Panel v2. Histologically, the tumor had two components: a central area of complex glandular proliferation with solid and cribriform architectures and a peripheral area showing adipocytic differentiation with spindle cell proliferation. Immunohistochemical staining revealed positive immunoreactivity for cytokeratin 5/6, epidermal growth factor receptor, and smooth muscle actin in both the central and peripheral areas. Next-generation sequencing-based genetic analysis identified a homozygous missense mutation (NM_004333.4:c.1742A>G; p.N581S) in the v-Raf murine sarcoma viral oncogene homolog B (BRAF) gene. Conclusion: We report the first case, as far as we are aware, of primary mammary adenoid cystic carcinoma with adipocytic differentiation. A novel missense mutation in BRAF was detected. The potential response to BRAF inhibitors in the presence of this mutation remains a matter for further studies.",
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Clinicopathological and molecular characteristics of mammary adenoid cystic carcinoma with adipocytic differentiation with emphasis on the identification of a novel BRAF mutation. / Bae, Go Eun; Yoon, Nara; Cho, Eun Yoon; Kim, Hyun Soo; Cho, Soo Youn.

In: Anticancer research, Vol. 39, No. 1, 01.2019, p. 369-374.

Research output: Contribution to journalArticle

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