Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips

Jaehoon Chung, Tom Bersano-Begey, Ken Pienta, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages21-23
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Fingerprint

Cell culture
Microfluidics
Culture Media
Assays
Gravitation
Cells
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chung, J., Bersano-Begey, T., Pienta, K., & Yoon, E. (2009). Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 21-23). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.
Chung, Jaehoon ; Bersano-Begey, Tom ; Pienta, Ken ; Yoon, Euisik. / Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 21-23 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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Chung, J, Bersano-Begey, T, Pienta, K & Yoon, E 2009, Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 21-23, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 09/11/1.

Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips. / Chung, Jaehoon; Bersano-Begey, Tom; Pienta, Ken; Yoon, Euisik.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 21-23 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.

AB - We present a single cell assay microfluidic chip that can 1) load cells into a microwell array at single cell resolution with a high capturing efficiency, 2) grow them into homogeneous clonal colonies without cell migration between neighbouring microwells, and 3) supply cell culture media or reagents to each microwell from a perfusion gravity flow induced by pressure difference between inlet and outlet reservoirs. Using the fabricated chip, we successfully identified three clonal phenotypes in prostate cancer cells and observed the drug responsiveness varies in different subtypes.

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Chung J, Bersano-Begey T, Pienta K, Yoon E. Clonal culture and chemodrug assay of heterogeneous cells (PC3 prostate carcinoma cells) using microfluidic single cell array chips. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 21-23. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).