Cloning and characterization of a divergent α-tubulin that is expressed specifically in dividing amebae of Naegleria gruberi

Sunglan Chung, Jaeyoung Cho, Hyeonjoo Cheon, Soonyoung Paik, Joohun Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A novel α-tubulin gene (α6) was cloned from a genomic library of Naegleria gruberi strain NB-1 and characterized. The open reading frame of α6 contained 1359 nucleotides encoding a protein of 452 amino acids (aa) with a calculated molecular weight of 50.5 kDa. The nucleotide sequence of the open reading frame of α6 showed considerable divergence (68.4% identity) when compared with previously cloned N. gruberi α-tubulin genes, which share about 97% identity in DNA sequences. The deduced aa sequence of α6-tubulin was 61.9% identical to that of α13-tubulin, which was cloned from the same strain, and showed similar identities to those of α-tubulins from other species (54∼62%). These data showed that α6-tubulin is one of the most divergent α-tubulins so far known. α6-tubulin was found to be expressed in actively growing cells and repressed quickly when these cells were induced to differentiate. Immunostaining with an antibody against α6-tubulin showed that α6-tubulin is present in the nuclei and mitotic spindle-fibers but absent in flagellar axonemes or cytoskeletal microtubules. These data finally established the presence of an α-tubulin that is specifically utilized for spindle-fiber microtubules and distinct from the flagellar axonemal α-tubulins in N. gruberi, hence confirmed the multi-tubulin hypothesis in this organism.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalGene
Volume293
Issue number1-2
DOIs
Publication statusPublished - 2002 Jun 26

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Naegleria
Amoeba
Tubulin
Organism Cloning
Microtubules
Open Reading Frames
Axoneme
Spindle Apparatus
Genomic Library

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Chung, Sunglan ; Cho, Jaeyoung ; Cheon, Hyeonjoo ; Paik, Soonyoung ; Lee, Joohun. / Cloning and characterization of a divergent α-tubulin that is expressed specifically in dividing amebae of Naegleria gruberi. In: Gene. 2002 ; Vol. 293, No. 1-2. pp. 77-86.
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Cloning and characterization of a divergent α-tubulin that is expressed specifically in dividing amebae of Naegleria gruberi. / Chung, Sunglan; Cho, Jaeyoung; Cheon, Hyeonjoo; Paik, Soonyoung; Lee, Joohun.

In: Gene, Vol. 293, No. 1-2, 26.06.2002, p. 77-86.

Research output: Contribution to journalArticle

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