Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens

Seobo Sim, Gab M. Park, Taisoon Yong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Clonorchis sinensis excretory-secretory (ES) antigens were separated by gradient SDS-PAGE and the antigenic protein isolated at 21 kDa was injected into rats. A C. sinensis cDNA library was then immunoscreened with sera from the 21-kDa antigenic protein-immunized rats. The selected genes, which were named C. sinensis myoglobin, contained a single open reading frame of 450 base pairs encoding 150 amino acids. A single hybridized band of 0.57 kb and two to four hybridized bands were detected by Northern and Southern blotting. Purified recombinant C. sinensis myoglobin was recognized by clonorchiasic rabbit sera (50%) and clonorchiasic human sera (25%). An indirect immunofluorescence assay showed that C. sinensis myoglobin is distributed over the whole body of the adult worm. It is believed that the abundance of C. sinensis myoglobin plays an important role as an oxygen reservoir under anaerobic conditions.

Original languageEnglish
Pages (from-to)338-343
Number of pages6
JournalParasitology Research
Volume91
Issue number4
DOIs
Publication statusPublished - 2003 Oct 1

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Clonorchis sinensis
myoglobin
Myoglobin
antiserum
Organism Cloning
Immune Sera
molecular cloning
antigens
Antigens
blood serum
Serum
rats
Indirect Fluorescent Antibody Technique
Southern Blotting
Gene Library
anaerobic conditions
cDNA libraries
Northern blotting
Base Pairing
Northern Blotting

All Science Journal Classification (ASJC) codes

  • Parasitology

Cite this

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Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens. / Sim, Seobo; Park, Gab M.; Yong, Taisoon.

In: Parasitology Research, Vol. 91, No. 4, 01.10.2003, p. 338-343.

Research output: Contribution to journalArticle

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