Cloning and characterization of NtTMK1 gene encoding a TMK1-homologous receptor-like kinase in tobacco

Hye Sun Cho, Hyunsook Pai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A cDNA encoding a receptor-like kinase, designated NtTMK1, was isolated from Nicotiana tabacum. The kinase domain of NtTMK1 contained all of 12 subdomains and invariant amino acid residues found in eukaryotic protein kinases. The extracellular domain contained 11 leucine-rich repeats which have been implicated in protein-protein interactions. The amino acid sequence of NtTMK1 exhibited high homology with those of TMK1 of Arabidopsis and TMK of rice in both kinase and extracellular domains, suggesting that NtTMK1 is a TMK homologue of tobacco. The NtTMK1 transcripts were present in all major plant organs, but its level varied in different developmental stages in anthers and floral organs. NtTMK1 mRNA accumulation in leaves was stimulated by CaCl2, methyl jasmonate, wounding, fungal elicitors, chitins, and chitosan. The NtTMK1 mRNA level also increased upon infection with tobacco mosaic virus.

Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalMolecules and cells
Volume10
Issue number3
Publication statusPublished - 2000 Jun 30

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Tobacco
Organism Cloning
Phosphotransferases
Genes
Tobacco Mosaic Virus
Messenger RNA
Chitin
Chitosan
Arabidopsis
Leucine
Protein Kinases
Amino Acid Sequence
Proteins
Complementary DNA
Amino Acids
Infection
Oryza
methyl jasmonate

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "A cDNA encoding a receptor-like kinase, designated NtTMK1, was isolated from Nicotiana tabacum. The kinase domain of NtTMK1 contained all of 12 subdomains and invariant amino acid residues found in eukaryotic protein kinases. The extracellular domain contained 11 leucine-rich repeats which have been implicated in protein-protein interactions. The amino acid sequence of NtTMK1 exhibited high homology with those of TMK1 of Arabidopsis and TMK of rice in both kinase and extracellular domains, suggesting that NtTMK1 is a TMK homologue of tobacco. The NtTMK1 transcripts were present in all major plant organs, but its level varied in different developmental stages in anthers and floral organs. NtTMK1 mRNA accumulation in leaves was stimulated by CaCl2, methyl jasmonate, wounding, fungal elicitors, chitins, and chitosan. The NtTMK1 mRNA level also increased upon infection with tobacco mosaic virus.",
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Cloning and characterization of NtTMK1 gene encoding a TMK1-homologous receptor-like kinase in tobacco. / Cho, Hye Sun; Pai, Hyunsook.

In: Molecules and cells, Vol. 10, No. 3, 30.06.2000, p. 317-324.

Research output: Contribution to journalArticle

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