Cloning and characterization of the full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase

Jae Won Jung, Won Sik Shin, Jihwan Song, Seung Taek Lee

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The 3.8-kb full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase was cloned by reverse transcription-PCR of mouse liver mRNA. The mouse PTK7 polypeptide showed 92.6% identity to human PTK7. The mouse Ptk7 gene consists of 20 exons and has exactly the same exon structure as the human PTK7 gene. Mouse PTK7 was shown to be phosphorylated neither by itself nor by other protein tyrosine kinases. In addition, its expression did not affect the phospho-tyrosine level of cellular proteins in COS-1 cells. The mouse Ptk7 mRNA was expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut, and craniofacial regions among embryonic tissues. These data suggest that mouse PTK7, an orthologue of human PTK7, plays multiple roles in embryonic development.

Original languageEnglish
Pages (from-to)75-84
Number of pages10
JournalGene
Volume328
Issue number1-2
DOIs
Publication statusPublished - 2004 Mar 17

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Receptor Protein-Tyrosine Kinases
Organism Cloning
Complementary DNA
Exons
Somites
Messenger RNA
COS Cells
Protein-Tyrosine Kinases
Genes
Reverse Transcription
Uterus
Embryonic Development
Tyrosine
Tail
Extremities
Polymerase Chain Reaction
Lung
Peptides
Liver
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "The 3.8-kb full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase was cloned by reverse transcription-PCR of mouse liver mRNA. The mouse PTK7 polypeptide showed 92.6{\%} identity to human PTK7. The mouse Ptk7 gene consists of 20 exons and has exactly the same exon structure as the human PTK7 gene. Mouse PTK7 was shown to be phosphorylated neither by itself nor by other protein tyrosine kinases. In addition, its expression did not affect the phospho-tyrosine level of cellular proteins in COS-1 cells. The mouse Ptk7 mRNA was expressed at high levels in lung and un-pregnant uterus among adult tissues, and in the tail, limbs, somites, gut, and craniofacial regions among embryonic tissues. These data suggest that mouse PTK7, an orthologue of human PTK7, plays multiple roles in embryonic development.",
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Cloning and characterization of the full-length mouse Ptk7 cDNA encoding a defective receptor protein tyrosine kinase. / Jung, Jae Won; Shin, Won Sik; Song, Jihwan; Lee, Seung Taek.

In: Gene, Vol. 328, No. 1-2, 17.03.2004, p. 75-84.

Research output: Contribution to journalArticle

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