Cloning and molecular characterization of groESL beat-shock operon in methylotrophic bacterium Methylovorus sp strain SS1 DSM 11726

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Abstract

The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SSI DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli sigma(32)-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SSI approximately 10min after increasing the temperature from 30 to 42 degrees C. The groESL operon was also induced by hydrogen peroxide or salt shock.
Original languageEnglish
Pages (from-to)695-702
Number of pages8
JournalBMB Reports
Volume38
Issue number6
Publication statusPublished - 2005

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Cloning
Molecular Cloning
Operon
Shock
Bacteria
Transcription Initiation Site
Escherichia coli
Hot Temperature
Chaperonin 10
Chaperonin 60
Messenger RNA
Molecular mass
Transcription
Genetic Promoter Regions
Hydrogen Peroxide
Temperature
Bacterial Proteins
Initiator Codon
Consensus Sequence
Salts

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@article{ecfc6b7933f148e393bb35f02ffe0b6e,
title = "Cloning and molecular characterization of groESL beat-shock operon in methylotrophic bacterium Methylovorus sp strain SS1 DSM 11726",
abstract = "The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SSI DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli sigma(32)-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SSI approximately 10min after increasing the temperature from 30 to 42 degrees C. The groESL operon was also induced by hydrogen peroxide or salt shock.",
author = "Eungbin KIm",
year = "2005",
language = "English",
volume = "38",
pages = "695--702",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "6",

}

TY - JOUR

T1 - Cloning and molecular characterization of groESL beat-shock operon in methylotrophic bacterium Methylovorus sp strain SS1 DSM 11726

AU - KIm, Eungbin

PY - 2005

Y1 - 2005

N2 - The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SSI DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli sigma(32)-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SSI approximately 10min after increasing the temperature from 30 to 42 degrees C. The groESL operon was also induced by hydrogen peroxide or salt shock.

AB - The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SSI DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli sigma(32)-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SSI approximately 10min after increasing the temperature from 30 to 42 degrees C. The groESL operon was also induced by hydrogen peroxide or salt shock.

M3 - Article

VL - 38

SP - 695

EP - 702

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 6

ER -