Cloning and molecular characterization of groESL heat-shock operon in methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726

Chi Yong Eom, Eungbin Kim, Young Tae Ro, Si Wouk Kim, Young Min Kim

Research output: Contribution to journalReview article

4 Citations (Scopus)

Abstract

The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichta coli σ32- type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10min after increasing the temperature from 30 to 42°C. The groESL operon was also induced by hydrogen peroxide or salt shock.

Original languageEnglish
Pages (from-to)695-702
Number of pages8
JournalJournal of Biochemistry and Molecular Biology
Volume38
Issue number6
Publication statusPublished - 2005 Nov 1

Fingerprint

Cloning
Molecular Cloning
Operon
Shock
Bacteria
Hot Temperature
Transcription Initiation Site
Chaperonin 10
Chaperonin 60
Messenger RNA
Molecular mass
Transcription
Genetic Promoter Regions
Escherichia coli
Hydrogen Peroxide
Temperature
Bacterial Proteins
Initiator Codon
Consensus Sequence
Salts

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

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title = "Cloning and molecular characterization of groESL heat-shock operon in methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726",
abstract = "The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichta coli σ32- type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10min after increasing the temperature from 30 to 42°C. The groESL operon was also induced by hydrogen peroxide or salt shock.",
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Cloning and molecular characterization of groESL heat-shock operon in methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726. / Eom, Chi Yong; Kim, Eungbin; Ro, Young Tae; Kim, Si Wouk; Kim, Young Min.

In: Journal of Biochemistry and Molecular Biology, Vol. 38, No. 6, 01.11.2005, p. 695-702.

Research output: Contribution to journalReview article

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T1 - Cloning and molecular characterization of groESL heat-shock operon in methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726

AU - Eom, Chi Yong

AU - Kim, Eungbin

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AU - Kim, Si Wouk

AU - Kim, Young Min

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N2 - The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichta coli σ32- type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10min after increasing the temperature from 30 to 42°C. The groESL operon was also induced by hydrogen peroxide or salt shock.

AB - The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichta coli σ32- type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10min after increasing the temperature from 30 to 42°C. The groESL operon was also induced by hydrogen peroxide or salt shock.

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