Cloning and sequence analysis of rhp51+, a Schizosaccharomyces pombe homolog of the Saccharomyces cerevisiae RAD51 gene

Yeun Kyu Jang, Yong Hwan Jin, Eun Mi Kim, Francis Fabre, Seung Hwan Hong, Sang Dai Park

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A homolog (rhp51+) of the RAD51 gene in Schizosaccharomyces pombe was cloned by screening a Sz. pombe genomic library using the 3'-end of RAD51 from Saccharomyces cerevisiae as a probe. As in S. cerevisiae, the sequence of rhp51+ showed two MluI cell-cycle boxes and a putative DNA damage-responsive element in its upstream region. The open reading frame codes for a 365-amino-acid (aa) polypeptide with an estimated molecular mass of 40 555 Da. The deduced aa sequence shows 27, 66, 75 and 80% identity with Escherichia coli RecA, S. cerevisiae Rad51 and the Rad51 homologs from chicken and humans, respectively. The aa sequence encoded by rhp51+ contains A- and B-type nucleotide-binding consensus sequences, as found in other RAD51 homologs. Northern blot analysis showed that rhp51+ encodes a 1.7-kb transcript. Methyl methanesulfonate treatment increased the level of this transcript three- to fivefold. Southern hybridization analysis suggests that a single copy of rhp51+ exists in the Sz. pombe genome.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalGene
Volume142
Issue number2
DOIs
Publication statusPublished - 1994 May 16

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Schizosaccharomyces
Sequence Analysis
Saccharomyces cerevisiae
Organism Cloning
Amino Acid Sequence
Genes
Methyl Methanesulfonate
Genomic Library
Consensus Sequence
Northern Blotting
Open Reading Frames
DNA Damage
Chickens
Cell Cycle
Nucleotides
Genome
Escherichia coli
Amino Acids
Peptides
Therapeutics

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Jang, Yeun Kyu ; Jin, Yong Hwan ; Kim, Eun Mi ; Fabre, Francis ; Hong, Seung Hwan ; Park, Sang Dai. / Cloning and sequence analysis of rhp51+, a Schizosaccharomyces pombe homolog of the Saccharomyces cerevisiae RAD51 gene. In: Gene. 1994 ; Vol. 142, No. 2. pp. 207-211.
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abstract = "A homolog (rhp51+) of the RAD51 gene in Schizosaccharomyces pombe was cloned by screening a Sz. pombe genomic library using the 3'-end of RAD51 from Saccharomyces cerevisiae as a probe. As in S. cerevisiae, the sequence of rhp51+ showed two MluI cell-cycle boxes and a putative DNA damage-responsive element in its upstream region. The open reading frame codes for a 365-amino-acid (aa) polypeptide with an estimated molecular mass of 40 555 Da. The deduced aa sequence shows 27, 66, 75 and 80{\%} identity with Escherichia coli RecA, S. cerevisiae Rad51 and the Rad51 homologs from chicken and humans, respectively. The aa sequence encoded by rhp51+ contains A- and B-type nucleotide-binding consensus sequences, as found in other RAD51 homologs. Northern blot analysis showed that rhp51+ encodes a 1.7-kb transcript. Methyl methanesulfonate treatment increased the level of this transcript three- to fivefold. Southern hybridization analysis suggests that a single copy of rhp51+ exists in the Sz. pombe genome.",
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Cloning and sequence analysis of rhp51+, a Schizosaccharomyces pombe homolog of the Saccharomyces cerevisiae RAD51 gene. / Jang, Yeun Kyu; Jin, Yong Hwan; Kim, Eun Mi; Fabre, Francis; Hong, Seung Hwan; Park, Sang Dai.

In: Gene, Vol. 142, No. 2, 16.05.1994, p. 207-211.

Research output: Contribution to journalArticle

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N2 - A homolog (rhp51+) of the RAD51 gene in Schizosaccharomyces pombe was cloned by screening a Sz. pombe genomic library using the 3'-end of RAD51 from Saccharomyces cerevisiae as a probe. As in S. cerevisiae, the sequence of rhp51+ showed two MluI cell-cycle boxes and a putative DNA damage-responsive element in its upstream region. The open reading frame codes for a 365-amino-acid (aa) polypeptide with an estimated molecular mass of 40 555 Da. The deduced aa sequence shows 27, 66, 75 and 80% identity with Escherichia coli RecA, S. cerevisiae Rad51 and the Rad51 homologs from chicken and humans, respectively. The aa sequence encoded by rhp51+ contains A- and B-type nucleotide-binding consensus sequences, as found in other RAD51 homologs. Northern blot analysis showed that rhp51+ encodes a 1.7-kb transcript. Methyl methanesulfonate treatment increased the level of this transcript three- to fivefold. Southern hybridization analysis suggests that a single copy of rhp51+ exists in the Sz. pombe genome.

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