Phospholipase C-β4(PLC-β4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-β4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-β1, human PLC-β2, and rat PLC-β3, respectively. The amino acid sequence of PLC-β4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-β subtypes, suggesting that the PLC-β4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 1993 Jul 30|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology