Cloning of cDNA Encoding Rat Phospholipase C-β4, a New Member of the Phospholipase C

Myung Jong Kim, Young Yil Bahk, Do Sik Min, Seung Jae Lee, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Phospholipase C-β4(PLC-β4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-β4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-β1, human PLC-β2, and rat PLC-β3, respectively. The amino acid sequence of PLC-β4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-β subtypes, suggesting that the PLC-β4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.

Original languageEnglish
Pages (from-to)706-712
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number2
DOIs
Publication statusPublished - 1993 Jul 30

Fingerprint

Cloning
Type C Phospholipases
Programmable logic controllers
Rats
Organism Cloning
Complementary DNA
Amino Acid Sequence
Amino Acids
Drosophila melanogaster
Gene Library
Cerebellum
Open Reading Frames
Isoenzymes
Drosophila
Molecular Weight
Brain
Molecular weight
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kim, Myung Jong ; Bahk, Young Yil ; Min, Do Sik ; Lee, Seung Jae ; Ryu, Sung Ho ; Suh, Pann Ghill. / Cloning of cDNA Encoding Rat Phospholipase C-β4, a New Member of the Phospholipase C. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 194, No. 2. pp. 706-712.
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abstract = "Phospholipase C-β4(PLC-β4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-β4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36{\%} identity with the sequences of rat PLC-β1, human PLC-β2, and rat PLC-β3, respectively. The amino acid sequence of PLC-β4, especially, shows higher identity (50{\%}) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-β subtypes, suggesting that the PLC-β4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.",
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Cloning of cDNA Encoding Rat Phospholipase C-β4, a New Member of the Phospholipase C. / Kim, Myung Jong; Bahk, Young Yil; Min, Do Sik; Lee, Seung Jae; Ryu, Sung Ho; Suh, Pann Ghill.

In: Biochemical and Biophysical Research Communications, Vol. 194, No. 2, 30.07.1993, p. 706-712.

Research output: Contribution to journalArticle

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