Antimicrobial resistance and multidrug resistance are slower-moving pandemics than the fast-spreading coronavirus disease 2019; however, they have potential to cause a much greater threat to global health. Here, we report a clustered regularly interspaced short palindromic repeats (CRISPR)-mediated surface-enhanced Raman scattering (SERS) assay for multidrug-resistant (MDR) bacteria. This assay was developed via a synergistic combination of the specific gene-recognition ability of the CRISPR system, superb sensitivity of SERS, and simple separation property of magnetic nanoparticles. This assay detects three multidrug-resistant (MDR) bacteria, species Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae, without purification or gene amplification steps. Furthermore, MDR A. baumannii-infected mice were successfully diagnosed using the assay. Finally, we demonstrate the on-site capture and detection of MDR bacteria through a combination of the three-dimensional nanopillar array swab and CRISPR-mediated SERS assay. This method may prove effective for the accurate diagnosis of MDR bacterial pathogens, thus preventing severe infection by ensuring appropriate antibiotic treatment.
Bibliographical noteFunding Information:
This research was supported by the Center for BioNano Health-Guard funded by the Ministry of Science and ICT of Korea (MSIT) as Global Frontier Project (H-GUARD_2013M3A6B2078950, H-GUARD_2014M3A6B2060507, and H-GUARD_2014M3A6B2060302), the Bio and Medical Technology Development Program of the National Research Foundation of Korea (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of NRF funded by MSIT (NRF-2019R1C1C1006867), and KRIBB Research initiative Program.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Physics and Astronomy(all)