CNS-specific ablation of steroidogenic factor 1 results in impaired female reproductive function

Ki Woo Kim, Shen Li, Hongyu Zhao, Boya Peng, Stuart A. Tobet, Joel K. Elmquist, Keith L. Parker, Liping Zhao

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The ventromedial hypothalamic nucleus (VMH) regulates a variety of homeostatic processes including female sexual behavior and reproduction. In the current study, we assessed the roles of steroidogenic factor 1 (SF-1) on reproductive function in the VMH using central nervous system-specific SF-1 knockout (SF-1 KOnCre;F/-) mice. Here we show that SF-1 KO nCre;F/- females exhibited marked impairment in female reproduction. Although male mice appeared to be normal in all aspects studied, including sexual behavior, SF-1 KOnCre;F/- females showed infertility or subfertility. Although adult SF-1 KOnCre;F/- females showed decreased or lacked corpora lutea, exogenous administration of gonadotropins induced the formation of multiple corpora lutea and induced normal ovulation, demonstrating that the ovaries are functionally intact. In addition, SF-1 KO nCre;F/- females stimulated with a synthetic GnRH agonist after priming exhibited markedly reduced LH secretion compared with wild-type littermates, arguing that disorganization in and around the VMH caused by SF-1 ablation interferes with the GnRH priming process or gonadotrope LH capacity. Furthermore, the SF-1 KOnCre;F/- females primed with estrogen benzoate and progesterone failed to induce steroid receptors around the VMH, consistent with impaired lordosis behavior in the SF-1 KOnCre;F/- females. Collectively, our results highlight that SF-1 in the VMH plays crucial roles in regulation of female reproductive function, presumably by organizing a precise neuronal connection and communication in and around the VMH.

Original languageEnglish
Pages (from-to)1240-1250
Number of pages11
JournalMolecular Endocrinology
Volume24
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1

Fingerprint

Steroidogenic Factor 1
Ventromedial Hypothalamic Nucleus
Female Infertility
Corpus Luteum
Gonadotropin-Releasing Hormone
Sexual Behavior
Reproduction
Lordosis
Steroid Receptors
Benzoates
Ovulation
Gonadotropins
Progesterone
Ovary
Estrogens

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

Cite this

Kim, Ki Woo ; Li, Shen ; Zhao, Hongyu ; Peng, Boya ; Tobet, Stuart A. ; Elmquist, Joel K. ; Parker, Keith L. ; Zhao, Liping. / CNS-specific ablation of steroidogenic factor 1 results in impaired female reproductive function. In: Molecular Endocrinology. 2010 ; Vol. 24, No. 6. pp. 1240-1250.
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Kim, KW, Li, S, Zhao, H, Peng, B, Tobet, SA, Elmquist, JK, Parker, KL & Zhao, L 2010, 'CNS-specific ablation of steroidogenic factor 1 results in impaired female reproductive function', Molecular Endocrinology, vol. 24, no. 6, pp. 1240-1250. https://doi.org/10.1210/me.2009-0206

CNS-specific ablation of steroidogenic factor 1 results in impaired female reproductive function. / Kim, Ki Woo; Li, Shen; Zhao, Hongyu; Peng, Boya; Tobet, Stuart A.; Elmquist, Joel K.; Parker, Keith L.; Zhao, Liping.

In: Molecular Endocrinology, Vol. 24, No. 6, 01.06.2010, p. 1240-1250.

Research output: Contribution to journalArticle

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