Co-autodisplay of Z-domains and bovine caseins on the outer membrane of E. Coli

Gu Yoo, Thorsten Saenger, Ji Hong Bong, Joachim Jose, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

In this work, two proteins, Z-domains and bovine casein, were autodisplayed on the outer membrane of the same Escherichia coli cells by co-transformation of two different autodisplay vectors. On the basis of SDS-PAGE densitometry, Z-domains and bovine casein were expressed at 3.12 × 105 and 1.55 × 105 proteins/E. Coli cell, respectively. The co-autodisplayed Z-domains had antibody-binding activity and the bovine casein had adhesive properties. E. Coli with co-autodisplayed proteins were analyzed by fluorescence assisted cell sorting (FACS). E. Coli with co-autodisplayed Z-domains and bovine casein aggregated due to hydrophobic interaction. For application to immunoassays, the Z-domain activity was estimated after (1) immobilizing the E. Coli and (2) forming an OM layer. E. Coli with co-autodisplayed two proteins that were immobilized on a polystyrene microplate had the same antibody-binding activity as did E. Coli with autodisplayed Z-domains only. The OM layer from the co-transformed E. Coli had Z-domains and bovine casein expressed at a 1:2 ratio from antibody-binding activity measurements.

Original languageEnglish
Pages (from-to)3126-3133
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1848
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

Bibliographical note

Funding Information:
This work was supported by DAPA and ADD . This research was supported by the National Research Foundation of Korea ( 2012R1A2A2A03047461 , 2014M3A9E5073818 and 2011-0020285 ), and by the Korea Small and Medium Business Administration ( S2220656 ).

Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

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