Co-autodisplay of Z-domains and bovine caseins on the outer membrane of E. Coli

Gu Yoo, Thorsten Saenger, Ji Hong Bong, Joachim Jose, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this work, two proteins, Z-domains and bovine casein, were autodisplayed on the outer membrane of the same Escherichia coli cells by co-transformation of two different autodisplay vectors. On the basis of SDS-PAGE densitometry, Z-domains and bovine casein were expressed at 3.12 × 105 and 1.55 × 105 proteins/E. Coli cell, respectively. The co-autodisplayed Z-domains had antibody-binding activity and the bovine casein had adhesive properties. E. Coli with co-autodisplayed proteins were analyzed by fluorescence assisted cell sorting (FACS). E. Coli with co-autodisplayed Z-domains and bovine casein aggregated due to hydrophobic interaction. For application to immunoassays, the Z-domain activity was estimated after (1) immobilizing the E. Coli and (2) forming an OM layer. E. Coli with co-autodisplayed two proteins that were immobilized on a polystyrene microplate had the same antibody-binding activity as did E. Coli with autodisplayed Z-domains only. The OM layer from the co-transformed E. Coli had Z-domains and bovine casein expressed at a 1:2 ratio from antibody-binding activity measurements.

Original languageEnglish
Pages (from-to)3126-3133
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1848
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

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Caseins
Escherichia coli
Membranes
Antibodies
Immobilized Proteins
Densitometry
Escherichia coli Proteins
Polystyrenes
Hydrophobic and Hydrophilic Interactions
Immunoassay
Sorting
Adhesives
Polyacrylamide Gel Electrophoresis
Proteins
Fluorescence
Cells

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Yoo, Gu ; Saenger, Thorsten ; Bong, Ji Hong ; Jose, Joachim ; Kang, Min Jung ; Pyun, Jae Chul. / Co-autodisplay of Z-domains and bovine caseins on the outer membrane of E. Coli. In: Biochimica et Biophysica Acta - Biomembranes. 2015 ; Vol. 1848, No. 12. pp. 3126-3133.
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Co-autodisplay of Z-domains and bovine caseins on the outer membrane of E. Coli. / Yoo, Gu; Saenger, Thorsten; Bong, Ji Hong; Jose, Joachim; Kang, Min Jung; Pyun, Jae Chul.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1848, No. 12, 01.12.2015, p. 3126-3133.

Research output: Contribution to journalArticle

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AU - Saenger, Thorsten

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AU - Pyun, Jae Chul

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