Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration

Sung Eun Kim, Young Pil Yun, Jae Yong Lee, June Sung Shim, Kyeongsoon Park, Jung Bo Huh

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The aim of this study was to improve osteoblast function by delivering two growth factors, PDGF-BB and BMP-2, incorporated onto heparinized titanium (Hep-Ti) substrate. To achieve co-delivery of PDGF-BB and BMP-2, the surface of anodized Ti was immobilized with heparin, and then the two growth factors were coated onto the Hep-Ti surface. Incorporation of the two growth factors onto Hep-Ti was evaluated by SEM and XPS. Incorporated PDGF-BB and BMP-2 were released from the Hep-Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on PDGF-BB- and BMP-2-immobilized Hep-Ti increased ALP activity, calcium deposition, osteocalcin and osteopontin levels as compared to those grown on PDGF-BB alone- or BMP-2 alone-immobilized Hep-Ti. These results suggested that co-delivery of PDGF-BB and BMP-2 using Hep-Ti substrate will be a promising material for the enhancement of osteoblast function and osteointegration.

Original languageEnglish
Pages (from-to)E219-E228
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Programme through the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (Grant No. 2012R1A1A005122), KBSI (Grant No. T32513, to KP), and the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (Grant No. A110416).

Publisher Copyright:
© 2015 John Wiley & Sons, Ltd.

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Co-delivery of platelet-derived growth factor (PDGF-BB) and bone morphogenic protein (BMP-2) coated onto heparinized titanium for improving osteoblast function and osteointegration'. Together they form a unique fingerprint.

Cite this