Co-digestion of untreated macro and microalgal biomass for biohydrogen production: Impact of inoculum augmentation and microbial insights

Periyasamy Sivagurunathan, Gopalakrishnan Kumar, Takuro Kobayashi, Kaiqin Xu, Sang Hyoun Kim, Dinh Duc Nguyen, Soon Woong Chang

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8 Citations (Scopus)


This study assessed the co-digestion of macro and microalgal biomass towards the improvement of hydrogen production. The red macroalgal biomass (Gelidium amansii) and green mixed microalgal biomass was mixed in a ratio of 8:2, with an initial substrate concentration of 10 g/L, and various amount of inoculum addition range from 3 to 15% (v/v) was evaluated to assess the feasible substrate to inoculum ratio for the effective co-digestion of the algal biomass. The results showed that the co-digestion with 6% inoculum addition provided the peak hydrogen yield of 45 mL/g dry biomass added with a high hydrogen content of 24% in the gas phase. The other tested conditions showed moderate hydrogen content in the range of 17–22%, respectively. These results suggest that anaerobic co-digestion of macro and microalgal biomass, with appropriate initial biomass loading (6%) is essential for enhanced hydrogen production.

Original languageEnglish
Pages (from-to)11484-11492
Number of pages9
JournalInternational Journal of Hydrogen Energy
Issue number25
Publication statusPublished - 2018 Jun 21

Bibliographical note

Funding Information:
The author Dr. Periyasamy Sivagurunathan greatly acknowledged the financial assistance from Japan Society for Promotion of Science: JSPS ID: P15370 for this study. This work was also supported by the Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953 ). This work was supported inpart by grants from the New & Renewable Energy Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (No. 20173010092470 ), the Korea Ministry of Environment , as a ‘Global Top Project’ (Project No. 2016002210003 ), and Institute for Information & communications Technology Promotion (IITP) grant funded by the Korea Government (MSIP) (Project No. 2017-0-00372 ).

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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