Co-immunization of plasmid DNA encoding IL-12 and IL-18 with bacillus Calmette-Guérin vaccine against progressive tuberculosis

Bo Young Jeon, Hyungjin Eoh, Sang Jun Ha, Hyeeun Bang, Seung Cheol Kim, Young Chul Sung, Sang Nae Cho

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Bacillus Calmette-Guérin (BCG) vaccine has widely been used to immunize against tuberculosis, but its protective efficacy is variable in adult pulmonary tuberculosis, while it is not efficiently protective against progressive infection of virulent Mycobacterium tuberculosis strains. In this study, the protective effects of plasmid DNA vaccine constructs encoding IL-12 or IL-18 with the BCG vaccine were evaluated against progressive infection of M. tuberculosis, using mouse aerosol challenge model. Materials and Methods: Plasmid DNA vaccine constructs encoding IL-12 or IL-18 were constructed and mice were immunized with the BCG vaccine or with IL-12 DNA or IL-18 DNA vaccine constructs together with the BCG vaccine. Results: The BCG vaccine induced high level of interferon gamma (IFN-γ) but co-immunization of IL-12 or IL-18 DNA vaccine constructs with the BCG vaccine induced significantly higher level of IFN-γ than a single BCG vaccine. The BCG vaccine was highly protective at early stage of M. tuberculosis infection, but its protective efficacy was reduced at later stage of infection. The co-immunization of IL-12 DNA vaccine constructs with the BCG vaccine was slightly more protective at early stage of infection and was significantly more protective at later stage infection than a single BCG vaccine. Conclusion: Co-immunization of IL-12 DNA vaccine with the BCG vaccine induced more protective immunity and was more effective for protection against progressive infection of M. tuberculosis.

Original languageEnglish
Pages (from-to)1008-1015
Number of pages8
JournalYonsei medical journal
Volume52
Issue number6
DOIs
Publication statusPublished - 2011 Nov 1

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BCG Vaccine
Interleukin-18
Interleukin-12
Bacillus
Immunization
Tuberculosis
Plasmids
DNA Vaccines
DNA
Mycobacterium tuberculosis
Infection
Interferon-gamma
Mycobacterium Infections
Aerosols
Pulmonary Tuberculosis
Immunity

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Jeon, Bo Young ; Eoh, Hyungjin ; Ha, Sang Jun ; Bang, Hyeeun ; Kim, Seung Cheol ; Sung, Young Chul ; Cho, Sang Nae. / Co-immunization of plasmid DNA encoding IL-12 and IL-18 with bacillus Calmette-Guérin vaccine against progressive tuberculosis. In: Yonsei medical journal. 2011 ; Vol. 52, No. 6. pp. 1008-1015.
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abstract = "Purpose: Bacillus Calmette-Gu{\'e}rin (BCG) vaccine has widely been used to immunize against tuberculosis, but its protective efficacy is variable in adult pulmonary tuberculosis, while it is not efficiently protective against progressive infection of virulent Mycobacterium tuberculosis strains. In this study, the protective effects of plasmid DNA vaccine constructs encoding IL-12 or IL-18 with the BCG vaccine were evaluated against progressive infection of M. tuberculosis, using mouse aerosol challenge model. Materials and Methods: Plasmid DNA vaccine constructs encoding IL-12 or IL-18 were constructed and mice were immunized with the BCG vaccine or with IL-12 DNA or IL-18 DNA vaccine constructs together with the BCG vaccine. Results: The BCG vaccine induced high level of interferon gamma (IFN-γ) but co-immunization of IL-12 or IL-18 DNA vaccine constructs with the BCG vaccine induced significantly higher level of IFN-γ than a single BCG vaccine. The BCG vaccine was highly protective at early stage of M. tuberculosis infection, but its protective efficacy was reduced at later stage of infection. The co-immunization of IL-12 DNA vaccine constructs with the BCG vaccine was slightly more protective at early stage of infection and was significantly more protective at later stage infection than a single BCG vaccine. Conclusion: Co-immunization of IL-12 DNA vaccine with the BCG vaccine induced more protective immunity and was more effective for protection against progressive infection of M. tuberculosis.",
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Co-immunization of plasmid DNA encoding IL-12 and IL-18 with bacillus Calmette-Guérin vaccine against progressive tuberculosis. / Jeon, Bo Young; Eoh, Hyungjin; Ha, Sang Jun; Bang, Hyeeun; Kim, Seung Cheol; Sung, Young Chul; Cho, Sang Nae.

In: Yonsei medical journal, Vol. 52, No. 6, 01.11.2011, p. 1008-1015.

Research output: Contribution to journalArticle

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T1 - Co-immunization of plasmid DNA encoding IL-12 and IL-18 with bacillus Calmette-Guérin vaccine against progressive tuberculosis

AU - Jeon, Bo Young

AU - Eoh, Hyungjin

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AU - Kim, Seung Cheol

AU - Sung, Young Chul

AU - Cho, Sang Nae

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N2 - Purpose: Bacillus Calmette-Guérin (BCG) vaccine has widely been used to immunize against tuberculosis, but its protective efficacy is variable in adult pulmonary tuberculosis, while it is not efficiently protective against progressive infection of virulent Mycobacterium tuberculosis strains. In this study, the protective effects of plasmid DNA vaccine constructs encoding IL-12 or IL-18 with the BCG vaccine were evaluated against progressive infection of M. tuberculosis, using mouse aerosol challenge model. Materials and Methods: Plasmid DNA vaccine constructs encoding IL-12 or IL-18 were constructed and mice were immunized with the BCG vaccine or with IL-12 DNA or IL-18 DNA vaccine constructs together with the BCG vaccine. Results: The BCG vaccine induced high level of interferon gamma (IFN-γ) but co-immunization of IL-12 or IL-18 DNA vaccine constructs with the BCG vaccine induced significantly higher level of IFN-γ than a single BCG vaccine. The BCG vaccine was highly protective at early stage of M. tuberculosis infection, but its protective efficacy was reduced at later stage of infection. The co-immunization of IL-12 DNA vaccine constructs with the BCG vaccine was slightly more protective at early stage of infection and was significantly more protective at later stage infection than a single BCG vaccine. Conclusion: Co-immunization of IL-12 DNA vaccine with the BCG vaccine induced more protective immunity and was more effective for protection against progressive infection of M. tuberculosis.

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