Co-transfection with cDNA encoding the Bcl family of anti-apoptotic proteins improves the efficiency of transfection in primary fetal neural stem cells

You Chan Kim, Jae Won Shim, Young J. Oh, Hyeon Son, Yong Sung Lee, Sang Hun Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Inefficiency in primary neural stem cell transfection is a major obstacle in gene expression research aimed at determining the mechanisms underlying brain development. Following our initial finding that liposome-mediated transfection procedures are fairly toxic to neural stem cells, we further examined whether transfection efficiency could be improved by preventing cells from undergoing apoptosis. Transfection efficiencies were markedly enhanced by co-transfection of cells with prototypic anti-apoptotic genes, such as bcl-2 and bcl-xL, and supplementing the culture medium with B27 Supplement. This combination of anti-apoptotic gene co-transfection and B27 Supplement resulted in approximately 5% transfection efficiency of primary neural stem cells, compared to less than 0.2% in control transfections. Therefore, this procedure and other similar approaches employed to enhance the efficiency of transfecting neural stem cells may facilitate the understanding of mechanisms underlying self-renewal of neural stem cells and their differentiation into various cell lineages.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalJournal of Neuroscience Methods
Volume117
Issue number2
DOIs
Publication statusPublished - 2002 Jun 30

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Fetal Stem Cells
Apoptosis Regulatory Proteins
Neural Stem Cells
Transfection
Complementary DNA
Poisons
Cell Lineage
Liposomes
Genes
Culture Media
Cell Differentiation
Apoptosis
Gene Expression

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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abstract = "Inefficiency in primary neural stem cell transfection is a major obstacle in gene expression research aimed at determining the mechanisms underlying brain development. Following our initial finding that liposome-mediated transfection procedures are fairly toxic to neural stem cells, we further examined whether transfection efficiency could be improved by preventing cells from undergoing apoptosis. Transfection efficiencies were markedly enhanced by co-transfection of cells with prototypic anti-apoptotic genes, such as bcl-2 and bcl-xL, and supplementing the culture medium with B27 Supplement. This combination of anti-apoptotic gene co-transfection and B27 Supplement resulted in approximately 5{\%} transfection efficiency of primary neural stem cells, compared to less than 0.2{\%} in control transfections. Therefore, this procedure and other similar approaches employed to enhance the efficiency of transfecting neural stem cells may facilitate the understanding of mechanisms underlying self-renewal of neural stem cells and their differentiation into various cell lineages.",
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Co-transfection with cDNA encoding the Bcl family of anti-apoptotic proteins improves the efficiency of transfection in primary fetal neural stem cells. / Kim, You Chan; Shim, Jae Won; Oh, Young J.; Son, Hyeon; Lee, Yong Sung; Lee, Sang Hun.

In: Journal of Neuroscience Methods, Vol. 117, No. 2, 30.06.2002, p. 153-158.

Research output: Contribution to journalArticle

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