Colanic acid is a novel phage receptor of Pectobacterium carotovorum subsp. carotovorum phage POP72

Hyeongsoon Kim, Minsik Kim, Jaewoo Bai, Jeong A. Lim, Sunggi Heu, Sangryeol Ryu

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The emergence and widespread nature of pathogen resistance to antibiotics and chemicals has led to the re-consideration of bacteriophages as an alternative biocontrol agent in several fields, including agriculture. In this study, we isolated and characterized a novel bacteriophage, POP72, that specifically infects Pectobacterium carotovorum subsp. carotovorum (Pcc), which frequently macerates agricultural crops. POP72 contains a 44,760 bp double-stranded DNA genome and belongs to the family Podoviridae. To determine the phage receptor for POP72, a random mutant library of Pcc was constructed using a Tn5 transposon and screened for resistance against POP72 infection. Most of the resistant clones had a Tn5 insertion in various genes associated with colanic acid (CA) biosynthesis. The phage adsorption rate and CA production decreased dramatically in the resistant clones. Complementation of the clones with the pUHE21-2 lacIq vector harboring genes associated with CA biosynthesis restored their sensitivity to POP72, as well as their ability to produce CA. These results suggest that CA functions as a novel phage receptor for POP72. The application of POP72 protected Chinese cabbage from Pcc infection, suggesting that phage POP72 would be an effective alternative antimicrobial agent to protect agricultural products from Pcc.

Original languageEnglish
Article number143
JournalFrontiers in Microbiology
Volume10
Issue numberFEB
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
This study received support from National Research Foundation of Korea (NRF) grant (NRF-2017R1A2A1A17069378). This work was also supported by the Yonsei University Future-leading Research Initiative of 2017 grant (2017-22-0060). HK was supported by the BK21 Plus Program of Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.

Publisher Copyright:
© 2019 Frontiers Media S.A. All Rights Reserved.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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