Cold atmospheric pressure plasma‐activated medium induces selective cell death in human hepatocellular carcinoma cells independently of singlet oxygen, hydrogen peroxide, nitric oxide and nitrite/nitrate

Yan Li, Tianyu Tang, Haejune Lee, Kiwon Song

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Abstract

Cold atmospheric pressure plasma (CAP) and plasma‐activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti‐cancer agents. The main components responsible for the selective anti‐cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti‐proliferative effect on Hep3B and Huh7 cells. The antiproliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N‐acetyl cysteine did not suppress PAM‐induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti‐cancer effects. The anti‐cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAMmediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti‐cancer effects in certain cancer cells.

Original languageEnglish
Article number5548
JournalInternational journal of molecular sciences
Volume22
Issue number11
DOIs
Publication statusPublished - 2021 Jun 1

Bibliographical note

Funding Information:
This research was supported by a grant (NRF2020R1A2C1102153) from the National Research Foundation (NRF) and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (MSIT) (NRF2016M3A9C6918275). Yan Li was partly supported by the post?BK program of the NRF of Korea.

Funding Information:
Funding: This research was supported by a grant (NRF2020R1A2C1102153) from the National Re‐ search Foundation (NRF) and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (MSIT) (NRF2016M3A9C6918275). Yan Li was partly supported by the post‐BK program of the NRF of Korea.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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