Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

Zhong Min Che, Tae Heob Jung, Jung Hee Choi, Do Jun Yoon, Hyun Jeong Jeong, Eun Ju Lee, Jin Kim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

Original languageEnglish
Pages (from-to)268-275
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number1
DOIs
Publication statusPublished - 2006 Jul 21

Fingerprint

Fibroblasts
Coculture Techniques
Cell culture
Cell Communication
Collagen
Cells
Neoplasms
Gels
Cathepsin D
Skin
Stromal Cells
Growth
Matrix Metalloproteinases
Tumors
Squamous Cell Carcinoma
Carcinogenesis
Cell Line

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Che, Zhong Min ; Jung, Tae Heob ; Choi, Jung Hee ; Yoon, Do Jun ; Jeong, Hyun Jeong ; Lee, Eun Ju ; Kim, Jin. / Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 346, No. 1. pp. 268-275.
@article{30a4a2dcda8940298481649c52083d27,
title = "Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction",
abstract = "The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.",
author = "Che, {Zhong Min} and Jung, {Tae Heob} and Choi, {Jung Hee} and Yoon, {Do Jun} and Jeong, {Hyun Jeong} and Lee, {Eun Ju} and Jin Kim",
year = "2006",
month = "7",
day = "21",
doi = "10.1016/j.bbrc.2006.05.111",
language = "English",
volume = "346",
pages = "268--275",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction. / Che, Zhong Min; Jung, Tae Heob; Choi, Jung Hee; Yoon, Do Jun; Jeong, Hyun Jeong; Lee, Eun Ju; Kim, Jin.

In: Biochemical and Biophysical Research Communications, Vol. 346, No. 1, 21.07.2006, p. 268-275.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction

AU - Che, Zhong Min

AU - Jung, Tae Heob

AU - Choi, Jung Hee

AU - Yoon, Do Jun

AU - Jeong, Hyun Jeong

AU - Lee, Eun Ju

AU - Kim, Jin

PY - 2006/7/21

Y1 - 2006/7/21

N2 - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

AB - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. Cathepsin D was also highly expressed when co-cultured with cancer cells and fibroblasts. The present study demonstrated that the presence of fibroblasts is essential in cancer invasion and that collagen gel-based co-culture models might be useful for invasive study.

UR - http://www.scopus.com/inward/record.url?scp=33744979500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744979500&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.05.111

DO - 10.1016/j.bbrc.2006.05.111

M3 - Article

VL - 346

SP - 268

EP - 275

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -