Collagen-binding motif peptide, a cleavage product of osteopontin, stimulates human neutrophil chemotaxis via pertussis toxin-sensitive G protein-mediated signaling

Mi Kyoung Kim, Sang Doo Kim, Ha Young Lee, Sun Young Lee, Jae Woong Shim, Jeanho Yun, Jong Min Kim, Do Sik Min, Young Hyun Yoo, Yoe Sik Bae

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of G i proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.

Original languageEnglish
Pages (from-to)3379-3384
Number of pages6
JournalFEBS Letters
Volume582
Issue number23-24
DOIs
Publication statusPublished - 2008 Oct 15

Fingerprint

Osteopontin
Pertussis Toxin
Chemotaxis
GTP-Binding Proteins
Neutrophils
Collagen
Peptides
Calcium
Extracellular Signal-Regulated MAP Kinases
Cell Surface Receptors
Mitogen-Activated Protein Kinases
Protein Kinases
Monocytes
Phosphotransferases
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Kim, Mi Kyoung ; Kim, Sang Doo ; Lee, Ha Young ; Lee, Sun Young ; Shim, Jae Woong ; Yun, Jeanho ; Kim, Jong Min ; Min, Do Sik ; Yoo, Young Hyun ; Bae, Yoe Sik. / Collagen-binding motif peptide, a cleavage product of osteopontin, stimulates human neutrophil chemotaxis via pertussis toxin-sensitive G protein-mediated signaling. In: FEBS Letters. 2008 ; Vol. 582, No. 23-24. pp. 3379-3384.
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abstract = "The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of G i proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.",
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Collagen-binding motif peptide, a cleavage product of osteopontin, stimulates human neutrophil chemotaxis via pertussis toxin-sensitive G protein-mediated signaling. / Kim, Mi Kyoung; Kim, Sang Doo; Lee, Ha Young; Lee, Sun Young; Shim, Jae Woong; Yun, Jeanho; Kim, Jong Min; Min, Do Sik; Yoo, Young Hyun; Bae, Yoe Sik.

In: FEBS Letters, Vol. 582, No. 23-24, 15.10.2008, p. 3379-3384.

Research output: Contribution to journalArticle

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AU - Kim, Mi Kyoung

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AU - Lee, Sun Young

AU - Shim, Jae Woong

AU - Yun, Jeanho

AU - Kim, Jong Min

AU - Min, Do Sik

AU - Yoo, Young Hyun

AU - Bae, Yoe Sik

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N2 - The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of G i proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.

AB - The collagen-binding motif (CBM) peptide, a cleavage product of osteopontin (OPN), stimulated intracellular calcium increase in human neutrophils. CBM peptide-stimulated calcium was inhibited by pertussis toxin (PTX), suggesting the influence of PTX-sensitive G-proteins. In addition CBM peptide stimulated the chemotactic migration of human neutrophils and human monocytes. CBM peptide-induced neutrophil chemotaxis was completely inhibited by PTX, once again indicating the influence of G i proteins. CBM peptide was also found to induce mitogen activated protein kinase activation. CBM peptide-induced neutrophil chemotaxis was mediated by p38 kinase as well as an extracellular signal-regulated protein kinase. Taken together, the results suggest that a cleavage product of OPN, CBM peptide, initiates immune responses by inducing neutrophil trafficking via certain PTX-sensitive cell surface receptors.

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