Collagen XVIII and corneal reinnervation following keratectomy

Tohru Sakimoto, Tae-im Kim, David Ellenberg, Naomi Fukai, Sandeep Jain, Dimitri T. Azar, Jin Hong Chang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The significance of collagen XVIII in the regulation of corneal reinnervation remains largely unknown. We used whole-mount immunoconfocal microscopy to localize collagen XVIII to the nerve basement membrane of wild-type (WT) mouse corneas. Transmission electron microscopy showed corneal nerve disorganization in collagen XVIII knockout mice (col18a1-/-). Antibody 2H3-specific neurofilament colocalized with collagens XVIII and IV and laminin-2 in WT mouse corneas, but did not colocalize with collagen IV and laminin-2 in col18a1-/- mouse corneas. Following keratectomy, col18a1-/- mice displayed decreased corneal neurite extension compared to WT mice. Our data indicate that collagen XVIII may play an important role in corneal reinnervation after wounding.

Original languageEnglish
Pages (from-to)3674-3680
Number of pages7
JournalFEBS Letters
Volume582
Issue number25-26
DOIs
Publication statusPublished - 2008 Oct 29

Fingerprint

Collagen Type XVIII
Cornea
Laminin
Intermediate Filaments
Neurites
Transmission Electron Microscopy
Basement Membrane
Knockout Mice
Microscopy
Microscopic examination
Collagen
Transmission electron microscopy
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Sakimoto, T., Kim, T., Ellenberg, D., Fukai, N., Jain, S., Azar, D. T., & Chang, J. H. (2008). Collagen XVIII and corneal reinnervation following keratectomy. FEBS Letters, 582(25-26), 3674-3680. https://doi.org/10.1016/j.febslet.2008.09.052
Sakimoto, Tohru ; Kim, Tae-im ; Ellenberg, David ; Fukai, Naomi ; Jain, Sandeep ; Azar, Dimitri T. ; Chang, Jin Hong. / Collagen XVIII and corneal reinnervation following keratectomy. In: FEBS Letters. 2008 ; Vol. 582, No. 25-26. pp. 3674-3680.
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Sakimoto, T, Kim, T, Ellenberg, D, Fukai, N, Jain, S, Azar, DT & Chang, JH 2008, 'Collagen XVIII and corneal reinnervation following keratectomy', FEBS Letters, vol. 582, no. 25-26, pp. 3674-3680. https://doi.org/10.1016/j.febslet.2008.09.052

Collagen XVIII and corneal reinnervation following keratectomy. / Sakimoto, Tohru; Kim, Tae-im; Ellenberg, David; Fukai, Naomi; Jain, Sandeep; Azar, Dimitri T.; Chang, Jin Hong.

In: FEBS Letters, Vol. 582, No. 25-26, 29.10.2008, p. 3674-3680.

Research output: Contribution to journalArticle

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Sakimoto T, Kim T, Ellenberg D, Fukai N, Jain S, Azar DT et al. Collagen XVIII and corneal reinnervation following keratectomy. FEBS Letters. 2008 Oct 29;582(25-26):3674-3680. https://doi.org/10.1016/j.febslet.2008.09.052