Collective dynamics of neuronal activities in various modular networks

Myung Uk Park, Yonghee Bae, Kyo Seok Lee, Jun Ho Song, Sun Mi Lee, Kyung Hwa Yoo

Research output: Contribution to journalArticlepeer-review

Abstract

Modularity is a key feature of structural and functional brain networks. However, the association between the structure and function of modular brain networks has not been revealed. We constructed three types of modular cortical networksin vitroand investigated their neuronal activities. The modular networks comprising 4, 3, or 2 modules were constructed using polydimethylsiloxane (PDMS) microstructures fabricated directly on a multi-electrode array (MEA) without transfer. The 4-module network had the strongest modular connectivity, followed by the 3-module and 2-module networks. To investigate how neuronal activities were affected by the modular network structure, spontaneous neuronal activities were recorded on different daysin vitroand analyzed based on spike amplitudes, network bursts, and the propagation properties of individual spikes. Different characteristics were observed depending on the network topology and modular connectivity. Moreover, when an electrode was stimulated by biphasic voltage pulses, bursts were elicited for the 4-module network, whereas spikes were elicited for the 3-module and 2-module networks. Direct fabrication of the PDMS microstructures on the MEA without transfer allows microscale construction of modular networks and high-density functional recording; therefore, the technique utilizing the PDMS microstructures can be applied to the systematic study of the dynamics of modular neuronal networksin vitro.

Original languageEnglish
Pages (from-to)951-961
Number of pages11
JournalLab on a chip
Volume21
Issue number5
DOIs
Publication statusPublished - 2021 Mar 7

Bibliographical note

Funding Information:
This work has been financially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, Future Planning (Grant No. 2020R1A202011942 and 2020R1A2C1006092).

Publisher Copyright:
© The Royal Society of Chemistry 2021.

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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