Colloidal gold nanoparticle conjugates of gefitinib

Anh Thu Ngoc Lam, Jinha Yoon, Erdene Ochir Ganbold, Dheeraj K. Singh, Doseok Kim, Kwang Hwi Cho, So Yeong Lee, Jaebum Choo, Kangtaek Lee, Sang Woo Joo

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13 Citations (Scopus)

Abstract

Gefitinib (GF) is a US Food and Drug Administration-approved epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor for treating the lung cancers. We fabricated colloidal gold nanoparticle (AuNP) conjugates of the GF anticancer drug by self-assembly to test their potency against A549, NCI-H460, and NCI-H1975 lung cancer cells. GF adsorption on AuNP surfaces was examined by UV-vis absorption spectra and surface-enhanced Raman scattering. Density functional theory calculations were performed to estimate the energetic stabilities of the drug-AuNP composites. The N1 nitrogen atom of the quinazoline ring of GF was calculated to be more stable than the N3 in binding Au cluster atoms. The internalizations of GF-coated AuNPs were examined by transmission electron and dark-field microscopy. A cell viability test of AuNP-GF conjugates with the EGFR antibody exhibited much higher reductions than free GF for A549, NCI-H460, and NCI-H1975 lung cancer cells after treatment for 48. h.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
Publication statusPublished - 2014 Nov 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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    Lam, A. T. N., Yoon, J., Ganbold, E. O., Singh, D. K., Kim, D., Cho, K. H., Lee, S. Y., Choo, J., Lee, K., & Joo, S. W. (2014). Colloidal gold nanoparticle conjugates of gefitinib. Colloids and Surfaces B: Biointerfaces, 123, 61-67. https://doi.org/10.1016/j.colsurfb.2014.08.021