Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells

Stephanie T. Shi, Jennifer J. Schiller, Amornrat Kanjanahaluethai, Susan C. Baker, Jong Won Oh, Michael M.C. Lai

Research output: Contribution to journalArticle

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Abstract

Murine hepatitis virus (MHV) gene 1, the 22-kb polymerase (pol) gene, is first translated into a polyprotein and subsequently processed into multiple proteins by viral autoproteases. Genetic complementation analyses suggest that the majority of the gene 1 products are required for viral RNA synthesis. However, there is no physical evidence supporting the association of any of these products with viral RNA synthesis. We have now performed immunofluorescent-staining studies with four polyclonal antisera to localize various MHV-A59 gene 1 products in virus-infected cells. Immunoprecipitation experiments showed that these antisera detected proteins representing the two papain-like proteases and the 3C-like protease encoded by open reading frame (ORF) 1a, the putative polymerase (p100) and a p35 encoded by ORF 1b, and their precursors. De novo-synthesized viral RNA was labeled with bromouridine triphosphate in lysolecithin-permeabilized MHV-infected cells. Confocal microscopy revealed that all of the viral proteins detected by these antisera colocalized with newly synthesized viral RNA in the cytoplasm, particularly in the perinuclear region of infected cells. Several cysteine and serine protease inhibitors, i.e., E64d, leupeptin, and zinc chloride, inhibited viral RNA synthesis without affecting the localization of viral proteins, suggesting that the processing of the MHV gene 1 polyprotein is tightly associated with viral RNA synthesis. Dual labeling with antibodies specific for cytoplasmic membrane structures showed that MHV gene 1 products and RNA colocalized with the Golgi apparatus in HeLa cells. However, in murine 17CL- 1 cells, the viral proteins and viral RNA did not colocalize with the Golgi apparatus but, instead, partially colocalized with the endoplasmic reticulum. Our results provide clear physical evidence that several MHV gene 1 products, including the proteases and the polymerase, are associated with the viral RNA replication-transcription machinery, which may localize to different membrane structures in different cell lines.

Original languageEnglish
Pages (from-to)5957-5969
Number of pages13
JournalJournal of Virology
Volume73
Issue number7
Publication statusPublished - 1999 Jun 23

Fingerprint

Murine hepatitis virus
Viral RNA
RNA
Membranes
Viral Proteins
Genes
viral proteins
genes
cells
antiserum
Polyproteins
Immune Sera
synthesis
proteinases
Golgi Apparatus
Golgi apparatus
Open Reading Frames
open reading frames
Peptide Hydrolases
Cytoplasmic Structures

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Shi, S. T., Schiller, J. J., Kanjanahaluethai, A., Baker, S. C., Oh, J. W., & Lai, M. M. C. (1999). Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells. Journal of Virology, 73(7), 5957-5969.
Shi, Stephanie T. ; Schiller, Jennifer J. ; Kanjanahaluethai, Amornrat ; Baker, Susan C. ; Oh, Jong Won ; Lai, Michael M.C. / Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells. In: Journal of Virology. 1999 ; Vol. 73, No. 7. pp. 5957-5969.
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Shi, ST, Schiller, JJ, Kanjanahaluethai, A, Baker, SC, Oh, JW & Lai, MMC 1999, 'Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells', Journal of Virology, vol. 73, no. 7, pp. 5957-5969.

Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells. / Shi, Stephanie T.; Schiller, Jennifer J.; Kanjanahaluethai, Amornrat; Baker, Susan C.; Oh, Jong Won; Lai, Michael M.C.

In: Journal of Virology, Vol. 73, No. 7, 23.06.1999, p. 5957-5969.

Research output: Contribution to journalArticle

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T1 - Colocalization and membrane association of murine hepatitis virus gene 1 products and de novo-synthesized viral RNA in infected cells

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AU - Schiller, Jennifer J.

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AU - Lai, Michael M.C.

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