Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Donghak Lee, Suho Ryu, Uihan Kim, Daeseong Jung, Chulmin Joo

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.

Original languageEnglish
Article number249958
Pages (from-to)4912-4922
Number of pages11
JournalBiomedical Optics Express
Volume6
Issue number12
DOIs
Publication statusPublished - 2015 Nov 18

Fingerprint

Microscopy
light emitting diodes
Color
microscopy
color
Light
gradients
Lighting
illumination
phase contrast
microscopes
sensors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Lee, Donghak ; Ryu, Suho ; Kim, Uihan ; Jung, Daeseong ; Joo, Chulmin. / Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging. In: Biomedical Optics Express. 2015 ; Vol. 6, No. 12. pp. 4912-4922.
@article{07f08fa5b0a04ad5a4759fab26d4b6d2,
title = "Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging",
abstract = "We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.",
author = "Donghak Lee and Suho Ryu and Uihan Kim and Daeseong Jung and Chulmin Joo",
year = "2015",
month = "11",
day = "18",
doi = "10.1364/BOE.6.004912",
language = "English",
volume = "6",
pages = "4912--4922",
journal = "Biomedical Optics Express",
issn = "2156-7085",
publisher = "The Optical Society",
number = "12",

}

Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging. / Lee, Donghak; Ryu, Suho; Kim, Uihan; Jung, Daeseong; Joo, Chulmin.

In: Biomedical Optics Express, Vol. 6, No. 12, 249958, 18.11.2015, p. 4912-4922.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

AU - Lee, Donghak

AU - Ryu, Suho

AU - Kim, Uihan

AU - Jung, Daeseong

AU - Joo, Chulmin

PY - 2015/11/18

Y1 - 2015/11/18

N2 - We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.

AB - We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.

UR - http://www.scopus.com/inward/record.url?scp=84961843102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961843102&partnerID=8YFLogxK

U2 - 10.1364/BOE.6.004912

DO - 10.1364/BOE.6.004912

M3 - Article

AN - SCOPUS:84961843102

VL - 6

SP - 4912

EP - 4922

JO - Biomedical Optics Express

JF - Biomedical Optics Express

SN - 2156-7085

IS - 12

M1 - 249958

ER -