Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles

Hosub Lee, Sang Woo Joo, So Yeong Lee, Chang Ha Lee, Kyong Ah Yoon, Kangtaek Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms.

Original languageEnglish
Pages (from-to)730-735
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number2
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Nucleotides
Polymorphism
Gold
Nanoparticles
Single Nucleotide Polymorphism
DNA
Agglomeration
Alleles
Thymine
Cytosine
Genotype
Suspensions
Oligonucleotide Probes
Oligonucleotides
DNA Probes
Color
Salts

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms.",
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Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles. / Lee, Hosub; Joo, Sang Woo; Lee, So Yeong; Lee, Chang Ha; Yoon, Kyong Ah; Lee, Kangtaek.

In: Biosensors and Bioelectronics, Vol. 26, No. 2, 01.10.2010, p. 730-735.

Research output: Contribution to journalArticle

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