Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples

S. N. Cho, G. M.E. Van Der Vliet, S. Park, S. H. Baik, S. K. Kim, Y. Chong, A. H.J. Kolk, P. R. Klatser, J. D. Kim

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Abstract

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

Original languageEnglish
Pages (from-to)752-754
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number3
Publication statusPublished - 1995 Jan 1

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Cho, S. N., Van Der Vliet, G. M. E., Park, S., Baik, S. H., Kim, S. K., Chong, Y., Kolk, A. H. J., Klatser, P. R., & Kim, J. D. (1995). Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples. Journal of Clinical Microbiology, 33(3), 752-754.