Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples

S. N. Cho, G. M.E. Van Der Vliet, S. Park, S. H. Baik, S. K. Kim, Y. Chong, A. H.J. Kolk, P. R. Klatser, J. D. Kim

Research output: Contribution to journalComment/debate

20 Citations (Scopus)

Abstract

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

Original languageEnglish
Pages (from-to)752-754
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number3
Publication statusPublished - 1995 Jan 1

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Sputum
Mycobacterium tuberculosis
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Cho, S. N., Van Der Vliet, G. M. E., Park, S., Baik, S. H., Kim, S. K., Chong, Y., ... Kim, J. D. (1995). Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples. Journal of Clinical Microbiology, 33(3), 752-754.
Cho, S. N. ; Van Der Vliet, G. M.E. ; Park, S. ; Baik, S. H. ; Kim, S. K. ; Chong, Y. ; Kolk, A. H.J. ; Klatser, P. R. ; Kim, J. D. / Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples. In: Journal of Clinical Microbiology. 1995 ; Vol. 33, No. 3. pp. 752-754.
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Cho, SN, Van Der Vliet, GME, Park, S, Baik, SH, Kim, SK, Chong, Y, Kolk, AHJ, Klatser, PR & Kim, JD 1995, 'Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples', Journal of Clinical Microbiology, vol. 33, no. 3, pp. 752-754.

Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples. / Cho, S. N.; Van Der Vliet, G. M.E.; Park, S.; Baik, S. H.; Kim, S. K.; Chong, Y.; Kolk, A. H.J.; Klatser, P. R.; Kim, J. D.

In: Journal of Clinical Microbiology, Vol. 33, No. 3, 01.01.1995, p. 752-754.

Research output: Contribution to journalComment/debate

TY - JOUR

T1 - Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples

AU - Cho, S. N.

AU - Van Der Vliet, G. M.E.

AU - Park, S.

AU - Baik, S. H.

AU - Kim, S. K.

AU - Chong, Y.

AU - Kolk, A. H.J.

AU - Klatser, P. R.

AU - Kim, J. D.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

AB - We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

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M3 - Comment/debate

C2 - 7751391

AN - SCOPUS:0028872247

VL - 33

SP - 752

EP - 754

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

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ER -