Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport

Eun Bi Choi, Jihye Choi, Seo Ryung Bae, Hyun Ouk Kim, Eunji Jang, Byunghoon Kang, Myeong Hoon Kim, Byeongyoon Kim, Jin Suck Suh, Kwangyeol Lee, Yong Min Huh, Seungjoo Haam

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitivity compared to pre-established trafficking agents employing one-dimensional turn-on of the fluorescence resonance-energy-transfer (FRET) signal. We fabricated polyaniline-based nanoprobes, which exhibited convertible transition states according to the proton levels, as an in situ indicator of vesicular transport pH. Silica-coated Fe3O4-MnO heterometal nanoparticles were synthesised and utilised as a metal oxidant to polymerise the aniline monomer. Finally, silica-coated polyaniline nanoparticles with adsorbed cyanine dye fluorophores Cy3 and Cy7 (FPSNICy3 and FPSNICy7) were fabricated as proton-sensitive nanoindicators. Owing to the selective quenching induced by the local pH variations of vesicular transport, FPSNICy3 and FPSNICy7 demonstrated excellent intracellular trafficking and provided sensitive optical indication of minute proton levels.

Original languageEnglish
Pages (from-to)1169-1179
Number of pages11
JournalNano Research
Volume8
Issue number4
DOIs
Publication statusPublished - 2015 Apr 1

Fingerprint

Polyaniline
Protons
Silicon Dioxide
Silica
Nanoprobes
Nanoparticles
Fluorophores
Cell death
Aniline
Oxidants
Metabolism
Quenching
Coloring Agents
Dyes
Monomers
Metals
Apoptosis
Oxidation-Reduction
polyaniline

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Electrical and Electronic Engineering

Cite this

Choi, Eun Bi ; Choi, Jihye ; Bae, Seo Ryung ; Kim, Hyun Ouk ; Jang, Eunji ; Kang, Byunghoon ; Kim, Myeong Hoon ; Kim, Byeongyoon ; Suh, Jin Suck ; Lee, Kwangyeol ; Huh, Yong Min ; Haam, Seungjoo. / Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport. In: Nano Research. 2015 ; Vol. 8, No. 4. pp. 1169-1179.
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Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport. / Choi, Eun Bi; Choi, Jihye; Bae, Seo Ryung; Kim, Hyun Ouk; Jang, Eunji; Kang, Byunghoon; Kim, Myeong Hoon; Kim, Byeongyoon; Suh, Jin Suck; Lee, Kwangyeol; Huh, Yong Min; Haam, Seungjoo.

In: Nano Research, Vol. 8, No. 4, 01.04.2015, p. 1169-1179.

Research output: Contribution to journalArticle

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AU - Choi, Jihye

AU - Bae, Seo Ryung

AU - Kim, Hyun Ouk

AU - Jang, Eunji

AU - Kang, Byunghoon

AU - Kim, Myeong Hoon

AU - Kim, Byeongyoon

AU - Suh, Jin Suck

AU - Lee, Kwangyeol

AU - Huh, Yong Min

AU - Haam, Seungjoo

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N2 - Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitivity compared to pre-established trafficking agents employing one-dimensional turn-on of the fluorescence resonance-energy-transfer (FRET) signal. We fabricated polyaniline-based nanoprobes, which exhibited convertible transition states according to the proton levels, as an in situ indicator of vesicular transport pH. Silica-coated Fe3O4-MnO heterometal nanoparticles were synthesised and utilised as a metal oxidant to polymerise the aniline monomer. Finally, silica-coated polyaniline nanoparticles with adsorbed cyanine dye fluorophores Cy3 and Cy7 (FPSNICy3 and FPSNICy7) were fabricated as proton-sensitive nanoindicators. Owing to the selective quenching induced by the local pH variations of vesicular transport, FPSNICy3 and FPSNICy7 demonstrated excellent intracellular trafficking and provided sensitive optical indication of minute proton levels.

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