Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells

Ying Mei, Krishanu Saha, Said R. Bogatyrev, Jing Yang, Andrew L. Hook, Z. Ilke Kalcioglu, Seung Woo Cho, Maisam Mitalipova, Neena Pyzocha, Fredrick Rojas, Krystyn J. Van Vliet, Martyn C. Davies, Morgan R. Alexander, Robert Langer, Rudolf Jaenisch, Daniel G. Anderson

Research output: Contribution to journalArticle

387 Citations (Scopus)

Abstract

Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure-function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high acrylate content, have a moderate wettability and employ integrin αvβ3 and αvβ5 engagement with adsorbed vitronectin to promote colony formation. The structure-function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.

Original languageEnglish
Pages (from-to)768-778
Number of pages11
JournalNature materials
Volume9
Issue number9
DOIs
Publication statusPublished - 2010 Sep

Fingerprint

stem cells
Biocompatible Materials
Stem cells
Biomaterials
Substrates
wettability
Cell culture
Wetting
Materials properties
Vitronectin
feeders
Surface topography
acrylates
Surface chemistry
indentation
stems
Indentation
Integrins
manipulators
modulus of elasticity

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Mei, Y., Saha, K., Bogatyrev, S. R., Yang, J., Hook, A. L., Kalcioglu, Z. I., ... Anderson, D. G. (2010). Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nature materials, 9(9), 768-778. https://doi.org/10.1038/nmat2812
Mei, Ying ; Saha, Krishanu ; Bogatyrev, Said R. ; Yang, Jing ; Hook, Andrew L. ; Kalcioglu, Z. Ilke ; Cho, Seung Woo ; Mitalipova, Maisam ; Pyzocha, Neena ; Rojas, Fredrick ; Van Vliet, Krystyn J. ; Davies, Martyn C. ; Alexander, Morgan R. ; Langer, Robert ; Jaenisch, Rudolf ; Anderson, Daniel G. / Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. In: Nature materials. 2010 ; Vol. 9, No. 9. pp. 768-778.
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Mei, Y, Saha, K, Bogatyrev, SR, Yang, J, Hook, AL, Kalcioglu, ZI, Cho, SW, Mitalipova, M, Pyzocha, N, Rojas, F, Van Vliet, KJ, Davies, MC, Alexander, MR, Langer, R, Jaenisch, R & Anderson, DG 2010, 'Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells', Nature materials, vol. 9, no. 9, pp. 768-778. https://doi.org/10.1038/nmat2812

Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. / Mei, Ying; Saha, Krishanu; Bogatyrev, Said R.; Yang, Jing; Hook, Andrew L.; Kalcioglu, Z. Ilke; Cho, Seung Woo; Mitalipova, Maisam; Pyzocha, Neena; Rojas, Fredrick; Van Vliet, Krystyn J.; Davies, Martyn C.; Alexander, Morgan R.; Langer, Robert; Jaenisch, Rudolf; Anderson, Daniel G.

In: Nature materials, Vol. 9, No. 9, 09.2010, p. 768-778.

Research output: Contribution to journalArticle

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AU - Kalcioglu, Z. Ilke

AU - Cho, Seung Woo

AU - Mitalipova, Maisam

AU - Pyzocha, Neena

AU - Rojas, Fredrick

AU - Van Vliet, Krystyn J.

AU - Davies, Martyn C.

AU - Alexander, Morgan R.

AU - Langer, Robert

AU - Jaenisch, Rudolf

AU - Anderson, Daniel G.

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Mei Y, Saha K, Bogatyrev SR, Yang J, Hook AL, Kalcioglu ZI et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nature materials. 2010 Sep;9(9):768-778. https://doi.org/10.1038/nmat2812