Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

Fan Yang, Seung-Woo Cho, Sun Mi Son, Sarah P. Hudson, Said Bogatyrev, Lily Keung, Daniel S. Kohane, Robert Langer, Daniel G. Anderson

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

Original languageEnglish
Pages (from-to)1909-1914
Number of pages6
JournalBiomacromolecules
Volume11
Issue number8
DOIs
Publication statusPublished - 2010 Aug 9

Fingerprint

Stem cells
Hydrogels
Tissue regeneration
Extracellular Matrix Proteins
Scaffolds (biology)
Chemical analysis
Tissue engineering
Tissue
Proteins

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Yang, F., Cho, S-W., Son, S. M., Hudson, S. P., Bogatyrev, S., Keung, L., ... Anderson, D. G. (2010). Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D. Biomacromolecules, 11(8), 1909-1914. https://doi.org/10.1021/bm100357t
Yang, Fan ; Cho, Seung-Woo ; Son, Sun Mi ; Hudson, Sarah P. ; Bogatyrev, Said ; Keung, Lily ; Kohane, Daniel S. ; Langer, Robert ; Anderson, Daniel G. / Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D. In: Biomacromolecules. 2010 ; Vol. 11, No. 8. pp. 1909-1914.
@article{c09bf9475d6941e2a87de3724ab1d682,
title = "Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D",
abstract = "Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.",
author = "Fan Yang and Seung-Woo Cho and Son, {Sun Mi} and Hudson, {Sarah P.} and Said Bogatyrev and Lily Keung and Kohane, {Daniel S.} and Robert Langer and Anderson, {Daniel G.}",
year = "2010",
month = "8",
day = "9",
doi = "10.1021/bm100357t",
language = "English",
volume = "11",
pages = "1909--1914",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "8",

}

Yang, F, Cho, S-W, Son, SM, Hudson, SP, Bogatyrev, S, Keung, L, Kohane, DS, Langer, R & Anderson, DG 2010, 'Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D', Biomacromolecules, vol. 11, no. 8, pp. 1909-1914. https://doi.org/10.1021/bm100357t

Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D. / Yang, Fan; Cho, Seung-Woo; Son, Sun Mi; Hudson, Sarah P.; Bogatyrev, Said; Keung, Lily; Kohane, Daniel S.; Langer, Robert; Anderson, Daniel G.

In: Biomacromolecules, Vol. 11, No. 8, 09.08.2010, p. 1909-1914.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Combinatorial extracellular matrices for human embryonic stem cell differentiation in 3D

AU - Yang, Fan

AU - Cho, Seung-Woo

AU - Son, Sun Mi

AU - Hudson, Sarah P.

AU - Bogatyrev, Said

AU - Keung, Lily

AU - Kohane, Daniel S.

AU - Langer, Robert

AU - Anderson, Daniel G.

PY - 2010/8/9

Y1 - 2010/8/9

N2 - Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

AB - Embryonic stem cells (ESCs) are promising cell sources for tissue engineering and regenerative medicine. Scaffolds for ESC-based tissue regeneration should provide not only structural support, but also signals capable of supporting appropriate cell differentiation and tissue development. Extracellular matrix (ECM) is a key component of the stem cell niche in vivo and can influence stem cell fate via mediating cell attachment and migration, presenting chemical and physical cues, as well as binding soluble factors. Here we investigated the effects of combinatorial extracellular matrix proteins on controlled human ESC (hESC) differentiation. Varying ECM compositions in 3D markedly affects cell behavior, and optimal compositions of ECM hydrogels are identified that facilitate specific-lineage differentiation of stem cells. To our knowledge, this is the first combinatorial analysis of ECM hydrogels for their effects on hESC differentiation in 3D. The 3D matrices described herein may provide a useful platform for studying the interactive ECM signaling in influencing stem cell differentiation.

UR - http://www.scopus.com/inward/record.url?scp=77955568351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955568351&partnerID=8YFLogxK

U2 - 10.1021/bm100357t

DO - 10.1021/bm100357t

M3 - Article

C2 - 20614932

AN - SCOPUS:77955568351

VL - 11

SP - 1909

EP - 1914

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 8

ER -