Combined anti-adipogenic effects of hispidulin and p-synephrine on 3t3-l1 adipocytes

Dahae Lee, Hee Jae Kwak, Byoung Ha Kim, Seung Hyun Kim, Dong Wook Kim, Ki Sung Kang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Hispidulin is abundant in Arrabidaea chica, Crossostephium chinense, and Grindelia argentina, among others. p-Synephrine is the main phytochemical constituent of Citrus aurantium. It has been used in combination with various other phytochemicals to determine synergistic effects in studies involving human participants. However, there have been no reports comparing the anti-adipogenic effects of the combination of hispidulin and p-synephrine. The current study explores the anti-adipogenic effects of hispidulin alone and in combination with p-synephrine in a murine preadipocyte cell line, 3T3-L1. Co-treatment resulted in a greater inhibition of the formation of red-labeled lipid droplets than the hispidulin or p-synephrine-alone treatments. Co-treatment with hispidulin and p-synephrine also significantly inhibited adipogenic marker proteins, including Akt, mitogen-activated protein kinases, peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, glucocorticoid receptor, and CCAAT/enhancer-binding protein β. Although further studies are required to assess the effects of each drug on pharmacokinetic parameters, a combination treatment with hispidulin and p-synephrine may be a potential alternative strategy for developing novel anti-obesity drugs.

Original languageEnglish
Article number1764
JournalBiomolecules
Volume11
Issue number12
DOIs
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
Funding: This results was supported by “Regional Innovation Strategy (RIS)” through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (2021RIS-001). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (2019R1F1A1059173).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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